Keywords: dogs, liver diseases, hepatodystrophy, acid erythrograms, diagnostic methods, erythrocytes, leukocytes, erythron, anemia, blood.


The task of the work was to analyze the dynamics of changes in the state of erythron depending on the severity of the course of hepatodystrophy. The material for the research was 10 dogs in which acute liver failure was caused. To do this, they were orally administered a 50 % carbon tetrachloride (CCl4) emulsion by means of a probe at a dose of 0.3 ml/kg, 0.5 and 1 ml/kg of the animal's weight with an interval of 6 days. In the blood, acid resistance was determined (according to Gitelzon I.I., Torskov I.A.) and the population composition of erythrocytes (according to Sizova I. with co-authors, 1985). Analysis of the acid resistance of dog erythrocytes induced by 0.00015 N HCl solution in 0.85 % NaCl solution showed that the erythrogram has well-defined left and right parts with a main peak height of 21.0 % and a hemolysis duration of 7.5 minutes. This configuration of the erythrogram is natural, since fractionation of erythrocytes in the sucrose density gradient showed that their populations are: "young" – 69.13±1.0; "mature" – 24.5±0.84 and "old" – 6.37±0.35 %. With the development of hepatodystrophy, the erythrogram also changed. Complete hemolysis of erythrocytes occurred at the sixth minute. The height of the main peak of hemolysis was 3 % higher and was 24 % at the third and a half minute, compared to the beginning of the experiment. Such a difference in the form of erythrograms before and after CCl4 poisoning indicates a decrease in the number of "young" erythrocytes. Thus, after the introduction of CCl4 at a dose of 1.0 ml/kg of weight, the population of "young" erythrocytes decreased by 12.03 %, compared to clinically healthy dogs, which was due to an increase in "mature" – by 8.4 % and "old" – by 3.63 %. Thus, experimentally induced hepatodystrophy in dogs causes changes in peripheral blood. "Young" erythrocytes stop entering the bloodstream, as evidenced by changes in erythrograms and the population composition of peripheral blood erythrocytes. The age composition of erythrocytes in the blood of dogs significantly affects the character of acidic erythrograms, the optimal form of which can be obtained when using a 0.00015 N solution of hydrochloric acid in an isotonic sodium chloride solution prepared in bidistilled water (pH 6.95). Anemia in dogs with hepatodystrophy was accompanied by a decrease of 12.0 % in the number of "young" and an increase of 8.4 and 3.6 % in the populations of "mature" and "old" erythrocytes, respectively.


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How to Cite
SolovіovaL. M. (2023). ERYTHROCYTOPOESIS IN CANINE HEPATODYSTROPHY PATIENTS. Bulletin of Sumy National Agrarian University. The Series: Veterinary Medicine, (3(62), 92-100.