EFFECT OF PRESOWING TREATMENT ON AMYLASE ACTIVITY AND CARBOHYDRATE AND PROTEIN CONTENT IN WINTER RYE GRAIN
Keywords:
winter rye, presowing treatment, vitamin E, ubiquinone-10, paraoxybenzoic acid, methionine, MgSO4
Abstract
Winter rye is a promising crop for the Polissya region of Ukraine, so the search for and development of effective and safe approaches to stimulate the growth and development of this crop is relevant. The material of the study was the seeds of winter rye (Secale cereale L.) varieties Synthetic 38 and Zabava, and compositions of metabolically active substances: vitamin E (10-8 M), paraoxybenzoic acid (POBA) (0,001%), methionine (0,001%), ubiquinone-10 (10-8 M) and MgSO4 (0,001%), which were used in the following combinations – vitamin E+POBA+methionine, vitamin E+POBA+methionine+MgSO4, vitamin E+ubiquinone-10. Presowing treatment of seeds with the studied compositions was performed and research was carried out on the content of protein, starch and water-soluble sugars, as well as the activity of amylases in winter rye grains. An increase in the protein content of winter rye grain is observed when the investigated compositions of metabolically active substances are used. At the same time, the greatest increase compared to the control group occurs with the use of vitamin E+POBA+methionine and E+POBA+methionine+MgSO4 combinations. Also, under presowing treatment of seeds with combinations of metabolically active substances, an increase in starch content is observed. The compositions E+POBA+methionine+MgSO4 and vitamin E+ubiquinone-10 in winter rye grains of both studied varieties turned out to be the most effective. At the same time, the content of soluble mono- and disaccharides in winter rye grains decreases under the conditions of presowing seed treatment. At the same time, presowing seed treatment with the studied combinations of metabolically active substances did not affect amylase activity in winter rye grains of both varieties. Only in the grain of winter rye of the Zabava variety in the group of presowing treatment with the E+POBA+methionine composition, a significant decrease in total amylase activity is observed due to a decrease in β-amylase activity. The results of the conducted research may be of practical importance for the further study of the influence of these compositions on plant organisms in order to justify their use in crop production.
References
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15. Kunkulberga, D., Linina, A., Kronberga, A., Kokare, A. & Lenenkova, I. (2017). Grain quality of winter rye cultivars grown in Latvia. Conference Paper “Foodbalt 2017”. doi: 10.22616/foodbalt.2017.015.
16. Kurylenko, A. O., Kurylenko, O. V., Kuchmenko, O. B. & Havii, V. M. (2021). Vplyv peredposivnoi obrobky nasinnia kompozytsiiamy metabolichno aktyvnykh rechovyn na morfometrychni pokaznyky ozymoho zhyta v umovakh Polissia Ukrainy [The influence of pre-sowing treatment of seeds with the composition of metabolically active substances on the morphometric indicators of winter rye in the conditions of Polissia of Ukraine]. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Seriia: Ahronomiia i biolohiia, 4, 25-32 doi: 10.32845/agrobio.2021.4.4. (in Ukrainian).
17. Kurylenko, A. O., Kurylenko, O. V., Kuchmenko, O. B. & Havii, V. M. (2021). Vplyv peredposivnoi obrobky nasinnia kompozytsiiamy metabolichno aktyvnykh rechovyn na vmist fotosyntetychnykh pihmentiv u lystkakh roslyn ozymoho zhyta sortiv Syntetyk 38 i Zabava na riznykh etapakh ontohenezu [Vplyv predposivnoi obrobky nasinnia kompozytsiiamy metabolichno aktivnykh rechovyn na vmist fotosyntetychnykh pihmentiv u lystkakh roslyn ozymoho zhyta sortiv Syntetyk 38 i Zabava na riznykh etapakh ontohenezu]. East European Scientific Journal, 75(11), 11–16 doi: 10.31618/ESSA.2782-1994.2021.4.75.169. (in Ukrainian).
18. Kuzminyih, A.N. & Pashkova, G.I. (2016). Urozhaynost i kachestvo zerna ozimoy rzhi v zavisimosti ot primeneniya stimulyatorov rosta [Productivity and grain quality of winter rye depending on the use of growth stimulants]. Vestnik Mariyskogo Gosudarstvennogo Universiteta. Seriya «Selskohozyaystvennyie Nauki. Ekonomicheskie Nauki», 1(5), 26–29 (in Russian).
19. Liu, M., & Lu, S. (2016). Plastoquinone and Ubiquinone in Plants: Biosynthesis, Physiological Function and Metabolic Engineering. Frontiers in Plant Science, 7. doi: 10.3389/fpls.2016.01898
20. Lobiuc, A., Damian, C., Costica, N. & Leahu, A. (2017). Morphological and biochemical parameters in chemically elicited rye sprouts. Studia Universitatis “Vasile Goldiş”, Seria Ştiinţele Vieţii, 27(3), 157–162.
21. Maathuis, F. J. (2009). Physiological functions of mineral macronutrients. Current Opinion in Plant Biology, 12(3), 250–258. doi: 10.1016/j.pbi.2009.04.003
22. Miret, J. A., & Munné-Bosch, S. (2015). Redox signaling and stress tolerance in plants: a focus on vitamin E. Annals of the New York Academy of Sciences, 1340(1), 29–38. doi: 10.1111/nyas.12639
23. Mokrosnop, V. M. (2014). Functions of tocopherols in the cells of plants and other photosynthetic organisms. The Ukrainian Biochemical Journal, 86(5), 26–36. doi: 10.15407/ubj86.05.026.
24. Muralikrishna, G. & Nirmala, M. (2005). Cereal α-amylases – an overview. Carbohyd, 60(2), 163-173. doi: 10.1016/j.carbpol.2004.12.002.
25. Sharma, K., Singh, U., Sharma, P., Kumar, A. & Sharma, L. (2015). Seed treatments for sustainable agriculture – a review. Journal of Applied and Natural Science, 7(1), 521–539. doi: 10.31018/jans.v7i1.641
26. Skrypnik, L., Maslennikov, P., Novikova, A., & Kozhikin, M. (2021). Effect of Crude Oil on Growth, Oxidative Stress and Response of Antioxidative System of Two Rye (Secale cereale L.) Varieties. Plants, 10(1), 157. doi: 10.3390/plants10010157
27. Stahl, E., Hartmann, M., Scholten, N., & Zeier, J. (2019). A Role for Tocopherol Biosynthesis in Arabidopsis Basal Immunity to Bacterial Infection. Plant Physiology, 181(3), 1008–1028. doi: 10.1104/pp.19.00618
28. Stepniewska, S., Cacak-Pietrzak, G., Szafranska, A., Ostrowska-Ligeza, E. & Dziki, D. (2021). Assessment of the starch-amylolytic complex of rye flours by traditional methofs and modern one. Materials, 14, 7603. doi: 10.3390/ma14247603
29. Szczepanek, M. (2018). Technology of maize with growth stimulants application. Engineering for Rural Development, 17, 483–490. doi: 10.22616/ERDev2018.17.N074
30. Yakhin, O. I., Lubyanov, A. A., Yakhin, I. A., & Brown, P. H. (2017). Biostimulants in plant science: A global perspective. Frontiers in Plant Science, 7. doi: 10.3389/fpls.2016.02049
31. Zymaroieva, A., Zhukov, O., Romanchuck, L., & Pinkin, A. (2019). Spatiotemporal dynamics of cereals grains and grain legumes yield in Ukraine. Bulgarian Journal of Agricultural Science, 25(6), 1107–1113.
2. Antonets, O. A. & Shramenko, K. I. (2021). Vplyv mineralnykh dobryv na urozhainist zerna zhyta ozymoho [The influence of mineral fertilizers on the yield of winter rye grain]. Materialy XI naukovo-praktychnoi internet-konferentsii «Aktualni napriamky ta problemy u tekhnolohiiakh vyroshchuvannia produktsii roslynnytstva» / Redkol.: V.V. Hanhur (vidp. red.) ta in. Poltavskyi derzhavnyi arharnyi universytet, 131–133 (in Ukrainian).
3. Asami, T., & Nakagawa, Y. (2018). Preface to the Special Issue: Brief review of plant hormones and their utilization in agriculture. Journal of Pesticide Science, 43(3), 154–158. doi: 10.1584/jpestics.M18-02
4. Crusciol, C. A. C., Arruda, D. P., Fernandes, A. M., Antonangelo, J. A., Alleoni, L. R. F. & Nascimento, C. A. C. (2018). Methods and extractants to evaluate silicon availability for sugarcane. Scientific Reports, 8, 916. doi: 10.1038/s41598-018-19240-1 PMID: 29343823
5. Demchuk, N. (2019) Zhyto ozyme: tekhnolohiia vyroshchuvannia: obrobitok gruntu, dobryva, nasinnia, zakhyst ta zbyrannia [Winter rye: cultivation technology: tillage, fertilizers, seeds, protection and harvesting]. Access mode: http://superagronom.com/articles/378-jito-ozime-tehnologiya-viroschuvannya-obrobitok-gruntu-dobriva-nasinnya-zahist-tazbirannya (in Ukrainian).
6. Furanets, O. A. & Piddubniak, V. A. (2019). Vplyv strokiv i doz azotnykh dobryv na vrozhainist ozymoho zhyta v umovakh promyvnoho vodnoho rezhymu [The influence of periods and doses of nitrogen fertilizers on the yield of winter rye under the conditions of the washing water regime]. Tavriiskyi naukovyi visnyk, 110(1), 194–199 (in Ukrainian).
7. Grytsaenko, Z. M., Grytsaenko, A. O. & Karpenko, V. P. (2003) Metody biolohichnykh ta ahrokhimichnykh doslidzhen roslyn i hruntiv [Methods of biological and agrochemical studies of plants and soils]. NICHLAWA, Kyiv, 320. (in Ukrainian).
8. Hildebrandt, T. M., Nunes Nesi, A., Araújo, W. L., & Braun, H.-P. (2015). Amino Acid Catabolism in Plants. Molecular Plant, 8(11), 1563–1579. doi: 10.1016/j.molp.2015.09.005
9. Horobets, M., Chaika, T. & Krykunova, V. (2021). Influence of growth stimulants on the ontogenesis of spring barley (Hordeum Vulgare L.). Colloquium-journal, (7(94)), 41–42. doi: 10.24412/2520-6990-2021-794-41-42
10. Hospodarenko, H. M. & Ptashnyk, M. M. (2013). Vmist bilka ta krokhmaliu v zerni zhyta ozymoho zalezhno vid vydiv, norm i strokiv vnesennia dobryv [The content of protein and starch in winter rye grains depending on the species, norms and terms of fertilization]. Novitni ahrotekhnolohii, 1(1), 5–10 (in Ukrainian).
11. Jiang, K., & Asami, T. (2018). Chemical regulators of plant hormones and their applications in basic research and agriculture. Bioscience, Biotechnology, and Biochemistry, 82(8), 1265–1300. doi: 10.1080/09168451.2018.1462693
12. Kabashnikova, L. F., Kalituho, L. N. & Derevinskij, A. V. (2003). Kolichestvennyj analiz svobodnyh i svjazannyh uglevodov v odnoj naveske rastitel’noj tkani [Quantitative analysis of free and bound carbohydrates in one sample of plant tissue]. BGPU, Minsk, 22. (in Russian).
13. Korotkova, I. V., Horobets, M. V., & Chaika, T. O. (2021). Vplyv Stymuliatoriv Rostu Na Produktyvnist Sortiv Yachmeniu Yaroho [Influence of Growth Stimulators on Productivity of Spring Barley Varieties]. Visnyk Poltavskoi Derzhavnoi Ahrarnoi Akademii, 2, 20–30 (in Ukrainian). doi: 10.31210/visnyk2021.02.02
14. Kunah, O. M., Pakhomov, O. Y., Zymaroieva, А. А., Demchuk, N. I., Skupskyi, R. M., Bezuhla, L. S., & Vladyka, Y. P. (2018). Agroeconomic and agroecological aspects of spatial variation of rye (Secale cereale) yields within Polesia and the Forest-Steppe zone of Ukraine: The usage of geographically weighted principal components analysis. Biosystems Diversity, 26(4), 276–285. doi: 10.15421/011842
15. Kunkulberga, D., Linina, A., Kronberga, A., Kokare, A. & Lenenkova, I. (2017). Grain quality of winter rye cultivars grown in Latvia. Conference Paper “Foodbalt 2017”. doi: 10.22616/foodbalt.2017.015.
16. Kurylenko, A. O., Kurylenko, O. V., Kuchmenko, O. B. & Havii, V. M. (2021). Vplyv peredposivnoi obrobky nasinnia kompozytsiiamy metabolichno aktyvnykh rechovyn na morfometrychni pokaznyky ozymoho zhyta v umovakh Polissia Ukrainy [The influence of pre-sowing treatment of seeds with the composition of metabolically active substances on the morphometric indicators of winter rye in the conditions of Polissia of Ukraine]. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Seriia: Ahronomiia i biolohiia, 4, 25-32 doi: 10.32845/agrobio.2021.4.4. (in Ukrainian).
17. Kurylenko, A. O., Kurylenko, O. V., Kuchmenko, O. B. & Havii, V. M. (2021). Vplyv peredposivnoi obrobky nasinnia kompozytsiiamy metabolichno aktyvnykh rechovyn na vmist fotosyntetychnykh pihmentiv u lystkakh roslyn ozymoho zhyta sortiv Syntetyk 38 i Zabava na riznykh etapakh ontohenezu [Vplyv predposivnoi obrobky nasinnia kompozytsiiamy metabolichno aktivnykh rechovyn na vmist fotosyntetychnykh pihmentiv u lystkakh roslyn ozymoho zhyta sortiv Syntetyk 38 i Zabava na riznykh etapakh ontohenezu]. East European Scientific Journal, 75(11), 11–16 doi: 10.31618/ESSA.2782-1994.2021.4.75.169. (in Ukrainian).
18. Kuzminyih, A.N. & Pashkova, G.I. (2016). Urozhaynost i kachestvo zerna ozimoy rzhi v zavisimosti ot primeneniya stimulyatorov rosta [Productivity and grain quality of winter rye depending on the use of growth stimulants]. Vestnik Mariyskogo Gosudarstvennogo Universiteta. Seriya «Selskohozyaystvennyie Nauki. Ekonomicheskie Nauki», 1(5), 26–29 (in Russian).
19. Liu, M., & Lu, S. (2016). Plastoquinone and Ubiquinone in Plants: Biosynthesis, Physiological Function and Metabolic Engineering. Frontiers in Plant Science, 7. doi: 10.3389/fpls.2016.01898
20. Lobiuc, A., Damian, C., Costica, N. & Leahu, A. (2017). Morphological and biochemical parameters in chemically elicited rye sprouts. Studia Universitatis “Vasile Goldiş”, Seria Ştiinţele Vieţii, 27(3), 157–162.
21. Maathuis, F. J. (2009). Physiological functions of mineral macronutrients. Current Opinion in Plant Biology, 12(3), 250–258. doi: 10.1016/j.pbi.2009.04.003
22. Miret, J. A., & Munné-Bosch, S. (2015). Redox signaling and stress tolerance in plants: a focus on vitamin E. Annals of the New York Academy of Sciences, 1340(1), 29–38. doi: 10.1111/nyas.12639
23. Mokrosnop, V. M. (2014). Functions of tocopherols in the cells of plants and other photosynthetic organisms. The Ukrainian Biochemical Journal, 86(5), 26–36. doi: 10.15407/ubj86.05.026.
24. Muralikrishna, G. & Nirmala, M. (2005). Cereal α-amylases – an overview. Carbohyd, 60(2), 163-173. doi: 10.1016/j.carbpol.2004.12.002.
25. Sharma, K., Singh, U., Sharma, P., Kumar, A. & Sharma, L. (2015). Seed treatments for sustainable agriculture – a review. Journal of Applied and Natural Science, 7(1), 521–539. doi: 10.31018/jans.v7i1.641
26. Skrypnik, L., Maslennikov, P., Novikova, A., & Kozhikin, M. (2021). Effect of Crude Oil on Growth, Oxidative Stress and Response of Antioxidative System of Two Rye (Secale cereale L.) Varieties. Plants, 10(1), 157. doi: 10.3390/plants10010157
27. Stahl, E., Hartmann, M., Scholten, N., & Zeier, J. (2019). A Role for Tocopherol Biosynthesis in Arabidopsis Basal Immunity to Bacterial Infection. Plant Physiology, 181(3), 1008–1028. doi: 10.1104/pp.19.00618
28. Stepniewska, S., Cacak-Pietrzak, G., Szafranska, A., Ostrowska-Ligeza, E. & Dziki, D. (2021). Assessment of the starch-amylolytic complex of rye flours by traditional methofs and modern one. Materials, 14, 7603. doi: 10.3390/ma14247603
29. Szczepanek, M. (2018). Technology of maize with growth stimulants application. Engineering for Rural Development, 17, 483–490. doi: 10.22616/ERDev2018.17.N074
30. Yakhin, O. I., Lubyanov, A. A., Yakhin, I. A., & Brown, P. H. (2017). Biostimulants in plant science: A global perspective. Frontiers in Plant Science, 7. doi: 10.3389/fpls.2016.02049
31. Zymaroieva, A., Zhukov, O., Romanchuck, L., & Pinkin, A. (2019). Spatiotemporal dynamics of cereals grains and grain legumes yield in Ukraine. Bulgarian Journal of Agricultural Science, 25(6), 1107–1113.
Published
2022-12-04
How to Cite
Kurylenko, A., & Kuchmenko, O. (2022). EFFECT OF PRESOWING TREATMENT ON AMYLASE ACTIVITY AND CARBOHYDRATE AND PROTEIN CONTENT IN WINTER RYE GRAIN. Bulletin of Sumy National Agrarian University. The Series: Agronomy and Biology, 48(2), 105-110. https://doi.org/10.32845/agrobio.2022.2.15
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