INFLUENCE OF PROBIOTIC DRUGS ON GROWTH INTENSITY AND HEMATOLOGICAL INDICATORS OF CARP
Abstract
The scientific article describes the effect of probiotic preparations on growth intensity and hematological indicators of carp. The question of stimulation of the innate immunity of carp with the help of natural and synthetic immunomodulators, as well as diversified programs of immunological protection in aquaculture, is considered. The aim of the work is to determine the effect of a probiotic preparation from L. Plantarum on the biochemical and linear parameters of carp. The object of the study is one-year-old scaly carp of the Ukrainian Nivkiv selection. The subject of the study is the weight of fish specimens, linear body measurements, biochemical indicators of blood, live weight gain. To fulfill the set goal, it was necessary to: monitor linear and mass changes of carp; to calculate the average arithmetic indicators of body weight according to different experimental groups; conduct biochemical studies of the blood of carp; formulate conclusions regarding the expediency of using probiotics. Scientific novelty. Combined feed with the addition of L. Plantarum probiotic and nanoselenium-containing probiotic was used to feed one-year-old carp in order to study the influence of diet composition on growth rates and biochemical blood parameters. The best results in terms of live mass dynamics were obtained from the research group whose diet included selenium nanoparticles and a probiotic preparation. During the analysis of blood indicators in the studied groups, a similar picture was revealed before the optimization of the metabolic and antioxidant status. It was established that the addition of nanoselenium in combination with probiotics reduces biomarkers of oxidative stress and lipid peroxidation, which optimizes metabolic parameters and reduces oxidative stress in fish. Practical significance of research. The maximum positive effect in obtaining fish planting material of carp of increased weight was observed when the probiotic L. Plantarum was introduced into the main diet in a complex with sodium selenite at the rate of 1 g per 1 kg of compound feed.
References
2. Bahdai T. (2016). Korop zvychainyi u vodnykh ekosystemakh ta akvakulturi [Common carp in aquatic ecosystems and aquaculture]. Lviv: Bulletin of the Lviv National Agrarian University. Series: Agronomy. № 20. Р. 182–186 (in Ukrainian).
3. Vovk N.I., Bozhyk V.I. (2014). Ikhtiopatolohiia [Ichthyopathology]. Kyiv: Р. 308 (in Ukrainian).
4. Hrynzhevskyi M.V. (2000). Intensyfikatsiia vyrobnytstva produktsii akvakultury u vnutrishnikh vodoimakh Ukrainy [Intensification of production of aquaculture products in internal water bodies of Ukraine]. Kyiv: World. Р. 188 (in Ukrainian).
5. Hrytsyniak I.I. (2010). Naukove zabezpechennia rozvytku akvakultury ta pidvyshchennia efektyvnosti vykorystannia vodnykh bioresursiv vnutrishnikh vodoim Ukrainy [Scientific support for the development of aquaculture and increasing the efficiency of the use of aquatic biological resources of inland water bodies of Ukraine]. Kyiv: Institute of Fisheries of the National Academy of Sciences. Fisheries science of Ukraine. № 1. Р. 4–13 (in Ukrainian).
6. Davydov O.M., Temnikhanov Yu.D. (2004). Osnovy veterynarno-sanitarnoho kontroliu u rybnytstvі [Basics of veterinary and sanitary control in fish farming]. Kyiv: «INKOS». Р. 144 (in Ukrainian).
7. Katiukha S.M., Orel A.M. (2018). Rol aboryhennykh ryb u vynyknenni invazii sered ryb-vselentsiv [The role of aboriginal fish in the emergence of invasions among resident fish]. Kyiv: Veterinary biotechnology. Vol. 32 (2). Р. 230–235 (in Ukrainian).
8. Kononenko R.V., Shevchenko P.H., Kondratiuk V.M., Kononenko I.S. (2016). Intensyvni tekhnolohii v akvakulturi [Intensive technologies in aquaculture]. Kyiv: «Center for educational literature». Р. 410 (in Ukrainian).
9. Kosiuk T.H., Hrynchuk Yu.Iu., Dmytruk I.V. (2016). Vyrobnytstvo i vykorystannia kombikormiv u hodivli ryb. Yakosti, bezpeky vyrobnytstva ta pererobky produktsii. [Production and use of compound feed in fish feeding. Quality, safety of production and processing of products]. Kyiv: Р. 94 (in Ukrainian).
10. Krushelnytska O.V., Loboiko Yu.V., Pukalo P.Ia., Kravets S.I. (2020). Sanitarno-gigiienichni doslidzhennia vody, gruntu ta kormu dlia ryb: navchalno-metodychnyi posibnyk [Sanitary and hygienic research of water, soil and feed for fish: educational and methodological guide]. Lviv: Р. 44 (in Ukrainian).
11. Mykytiuk P.V., Dzhmil V.I., Bukalova N.V. ta in. (2009). Praktykum z biolohii, patolohii ta vetsanekspertyzy prisnovodnoi ryby [Workshop on biology, pathology and veterinary expertise of freshwater fish]. Bila Tserkva: Р. 160 (in Ukrainian).
12. Sondak V.V., Hrytsyk O.B., Rud O.H. (2016). Invaziini khvoroby ryb: navchalnyi posibnyk [Invasive fish diseases: a study guide]. Rivne: НУВГП. Р. 145 (in Ukrainian).
13. Stybel V.V, Berezovskyi A.V., Dovhii Yu.Iu. ta in. (2016). Invaziini khvoroby ryb: navchalnyi posibnyk [Invasive fish diseases: a study guide]. Zhytomyr: Polissia. Р. 142 (in Ukrainian).
14. Sherman I.M., Hrynzhevskyi M.V., Zheltov Yu.O. (2001). Hodivlia ryb [Fish feeding]. Kyiv: Higher Education. Р. 269 (in Ukrainian).
15. Ahne W. (1983). Presence of infectious pancreatic necrosis virus in the seminal fluid of the rainbow trout, Salmo gairdneri Richardson. Journal of Fish Diseases № 6. Р. 377–378
16. Ahne W., Negele R.D. (1985). Studies on the transmission of infectious pancreatic necrosis virus. Journal of Fish Diseases № 29. Р. 61–65
17. Almendras J.M.E. (2001). Immunity and biological methods of disease prevention and control. G.D. Lio–Po, C.R. Lavilla, E.R. Cruz–Lacierda, Tigbauan, Iloilo, Philippines SEAFDEC Aquaculture Department: Health management in aquaculture (Eds). Р. 111–136
18. Aly S.M., Abb–Allah O., Mahmoud A., Gafer H. (2010). Efficiency of levamisole in improving the immune response of catfish (Clarias gariepenus) to Aeromonas hydrophila vaccine: clinico–pathological studies. Mediterranean Aquaculture Journal. Vol. 4 (1). Р. 18–26
19. Anderson D. (1992). Immunostimulants, adjuvants, and vaccine carriers in fish: applications to aquaculture. Annual Review of Fish Diseases. Vol. 2. Р. 281–307
20. Anderson D.P., Siwicki A.K. (1996). Pollutant exposures and drug treatments of fish: detection of the effects on the immune response and protection against diseases. Oxford & IBH Publishing Co: The Role of Aquaculture in World Fisheries. Р. 195–199
21. Bergmann S.M., Kempter J., Sadowski J., Fichter D. (2006). First detection, confirmation and isolation of koi herpesvirus (KHV) in cultured common carp (Cyprinus carpio L.) in Poland. Bulletin of the European Association of Fish Pathologists. Vol. 26 (2). Р. 97–104
22. Bogusawska E. (2015). The co–existence of microorganisms in biofilm as the basis for the functionality of biological deposits. Gdynia: Training Materials for the XL Salmonid Aquaculturist Conference (Ed.) A. Kowalska. Р. 57–66
23. Bowden T.J. (2008). Modulation of the immune system of fish by their environment. Fish Shellfish Immunol. Vol. 25. Р. 373–383
24. Brucka-Jastrzеbska E., Kawczuga D., Rajkowska M., Protasowicki M. (2009). Levels of microelements (Cu, Zn, Fe) and macroelements (Mg, Ca) in freshwater fish. Journal of Elementology. Vol. 14. Р. 437–447