DYNAMICS OF BIOCHEMICAL AND HORMONAL CHANGES IN THE BLOOD OF PREGNANT AND NON-PREGNANT COWS AFTER THE APPLICATION OF BIOLOGICALLY ACTIVE SUBSTANCES
Abstract
It is estimated that up to 50% of embryos die during the first and second weeks of pregnancy. In this regard, the interaction between the embryo and the mother's body is crucial for successful implantation, which is influenced by the hormonal and metabolic state of the female. The article presents the materials of our own research on the dynamics of biochemical and hormonal parameters of pregnant and non-pregnant cows after the introduction of nanocarboxylate complexes and the drug Quatronan-Se. The studies were performed on Simmental cows, nanocarboxylates were administered to animals on day 10-12 of the sexual cycle, and blood was taken on day 13. Studies have shown that the highest level of fertility was in the second experimental group, which injected the drug Quatronan-Se. According to the results, it was found that nanocarboxylates activate cholesterol synthesis because all groups injected nanocarboxylates had higher cholesterol levels and were within the physiological norm. The highest level of this indicator was in the II group , which is 10.6% and 7.1% higher compared to I, III. It should also be noted that in non-pregnant animals of group IV, which were administered nanocarboxylates the level of the studied indicator was significantly higher compared to the control group by 66.9% (p <0,01). It is known that glucose has a direct effect on embryo implantation, the results suggest that nanocarboxylates intensify carbohydrate metabolism, as in the experimental groups there is a higher level of glucose than in the control group. In our studies, the highest levels of this metabolite were in group II, which was 8.6%, 7.7%, 9.4% and 5.4% higher than in groups I, III, control and IV. In addition, a comparative analysis of the concentration of steroid hormones in the blood of pregnant and non-pregnant animals shows that in pregnant animals the concentration of progesterone was higher, in group II it was 4.28 ng / ml, which is 1.8% and 2.6% higher compared with the I and III experimental groups. In non-pregnant animals, progesterone levels were significantly lower. It was also found that nacocarboxylates do not have a significant effect on estradiol levels, as no significant changes in the study groups on the level of this indicator were detected.
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