Study of irritant and toxic effect of SUKHODEZ disinfectant

Daria  Slaston; Olena Kotsur; Tetiana Fotina

doi:10.32845/bsnau.vet.2020.3.2

Daria Slaston Sumy National Agrarian University https://orcid.org/0000-0002-8110-3647
Olena Kotsur Sumy National Agrarian University https://orcid.org/0000-0001-7779-9306
Tetiana Fotina Sumy National Agrarian University https://orcid.org/0000-0001-5079-2390

DOI: https://doi.org/10.32845/bsnau.vet.2020.3.2

Keywords: disinfection, disinfectant, laboratory, microorganisms, allergy, subraction

Abstract

The article presents the results of the study of irritant and allergenic action of the disinfectant "Sukhodez" in the laboratory. As a result, we can say that the drug Sukhodez has a weak effect on the degree of response to irritation in mice and rabbits. However, the drug in a single treatment of the premises in the maximum allowable concentration of 60 mg / m3 has a moderately irritating effect on the mucous membranes of animals, which allows it to be classified as hazard class 3.

The vast majority of powder disinfectants are of foreign origin. Therefore, the study and development of powder disinfectants for bedding in agriculture, in particular in poultry is currently quite relevant. According to the results of our study, there are currently no more than 9 manufacturers of disinfectants in agriculture in Ukraine. At the same time, representatives of disinfectants of foreign origin outnumber this number dozens of times.

We studied the toxicity of Sukhodes with long-term oral administration to two groups of white rats selected according to the principle of analogues.We also studied the effect of the drug on the condition of internal organs, which was assessed by comparing their relative mass ratios.

The ability of the drug to penetrate the skin was evaluated after the experiment. The clinical picture of animal intoxication and the effect on the central nervous system were taken into account. In addition, the method of "swimming" was used to assess the performance of mice. To do this, recorded the time during which the animal could stay on the surface of the water.

We did research on the local irritant effect of "Sukhodez". Determined by the method of skin applications, which allows to detect the development of mice in non-allergic contact dermatitis depending on the dose of the substance used.

The study of the toxicity of the drug "Sukhodes" with prolonged oral administration showed that the introduction of the drug at a dose of 2500 mg / kg body weight for 30 days did not affect the clinical condition and behavior of rats. No foreign or negative effects of Sukhodes were recorded throughout the experiment. Animals willingly consumed food and water, were mobile, responded adequately to sound and tactile stimuli.The studies revealed low toxicity of the drug.

Based on the research, we concluded that the tool "Sukhodez" does not cause the death of experimental rats and mice and it can be classified as hazard class 4 according to the International Standard GOST 12.1.007-76, or category 5 according to the International Global Classification Global Harmonized System, (GHS).

References

1. Derzhavnyi reiestr veterynarnykh preparativ, kormovykh dobavok, hotovykh kormiv ta premiksiv. [State register of veterinary drugs, feed additives, hot feed and premixes]. URL: http://vet.in.ua/menu/drugs.php?id_drugtype=7
2. Gilbert P. & Moore L. (2005). Cationic antiseptics: diversity of action under a common epithet . Journal of Applied Microbiology, 99(4), 703-715 doi:10.1111/j.1365-2672.2005.02664.x .
3. Lysytsia A., Stepaniak I., Mandyhra M. & Mandyhra Yu. (2008). Patent 34286 Ukraina, MPK (2006) A 61 K 33/00. Zasib dezinfikuiuchyi Epidez [Patent 34286 Ukraine, IPC (2006) A 61 K 33/00. Disinfectant Epidez]. Zaiavnyk i vlasnyk patentu Instytutu epizootolohii UAAN [applicant and patent owner of the Institute of Epizootology UAAS], 01694; zaiavl. 08.02.2008; opubl. 11.08.2008, Biul. № 15. (in Ukrainian)
4. Mandyhra M., Lysytsia A., Stepaniak I. et al., (2009). Perspektyvy vykorystannia polimernykh pokhidnykh huanidynu dlia dezinfektsii pry tuberkulozi [Prospects for the use of polymeric derivatives of guanidine for disinfection in tuberculosis]. Naukovo-tekhnichnyi biuleten Instytutu biolohii tvaryn i DNDKI vetpreparativ ta kormovykh dobavok [Scientific and technical bulletin of the Institute of Animal Biology and DNDKI veterinary drugs and feed additives], 10(4), 169-175. (in Ukrainian)
5. Mandyhra M., Serhiichyk S., Lysytsia A. et al. (2011). Patent na korysnu model № 63862 Zasib dezinfikuiuchyi stiikyi do zamerzannia «Epi-dez-barier» [Patent for utility model № 63862 Disinfectant resistant to freezing "Epidemic barrier"], Zaiavnyk i vlasnyk patentu Instytut epizootolohii NAAN [Applicant and patent owner of the Institute of Epizootology UAAS], zaiavl. 14.03.2011 zaiavka № u 201102987; opubl. 25.10.2011, Biul. № 20. (in Ukrainian)
6. Mandyhra M., Lysytsia A., Stepaniak I., et al., (2009). Porivnialna otsinka bakterytsydnoi aktyvnosti riznykh pokhidnykh huanidynu [Comparative evaluation of bactericidal activity of various guanidine derivatives ]. Naukovyi visnyk Lvivskoho nats. universytetu vet. medytsyny ta biotekhnolohii im. S. Gzhytskoho [Scientific Bulletin of the Lviv National University. University of Vet. of Medicine and Biotechnology S. Gzhytsky], 2 (41), 220-226. (in Ukrainian)
7. Romanishyna O, Mandyhra-Melnyk Yu., Mandyhra M., & Lysytsia A. (2010). Biotsydna diia polimernykh pokhidnykh huanidynu na kulturu leptospir [Biocidal effect of polymeric derivatives of guanidine on leptospira culture]. Naukovyi visnyk Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy [Scientific Bulletin of the National University of Life and Environmental Sciences of Ukraine], 151(2), 166-170. (in Ukrainian)
8. Spysok zareiestrovanykh veterynarnykh preparativ, kormovykh dobavok, hotovykh kormiv ta premiksiv [List of registered veterinary drugs, feed additives, finished feeds and premixes.]: URL: http://www.vet.gov.ua/sites/default/files/R_1_15.xl.s.
9. Zakon Ukrainy «Pro osnovni pryntsypy ta vymohy do bezpechnosti ta yakosti kharchovykh produktiv» vid 6 veresnia 2005 r. № 2809-IV. [Law of Ukraine "On Basic Principles and Requirements for Food Safety and Quality" of September 6, 2005 № 2809-IV.] URL: https://zakon.rada.gov.ua/laws/show/771/97-%D0%B2%D1%80#Text
10. Zavhorodnii A., Palii A., & Kalashnyk N. (2008). Bakterytsydni vlastyvosti dezinfektantu Delanol shchodo mikobakterii ta salmonel [Bactericidal properties of Delanol disinfectant against mycobacteria and salmonella]. Veterynarna medytsyna. Mizhvidomchyi tematychnyi naukovyi zbirnyk [Veterinary medicine. Interdepartmental thematic scientific collection], 91, 207-211. (in Ukrainian)
11. Fotina T., & Vashchyk, Y.(2017). Comparative evaluation of effectiveness of preparations “Saroflox” and enrofloxacin towards to the pathogens of poultry’s bacterial diseases. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 19(77), 143–147. doi:10.15421/nvlvet7731 .
12. HOST 12.1.007 - 76 SSBT. Shkidlyvi rechovyny. Klasyfikatsiia i zahalni vymohy bezpeky. [State standard 12.1.007 - 76 SSBT. Hazardous substances. Classification and general safety requirements].
13. Kasianenko O., Berezovskyi A., Kasianenko S., & Dolbonosova R. (2019). Analysis of the market of disinfectants in Ukraine. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 20(2), 439-445. doi:10.36359/scivp.2019-20-2.56
14. Khudiakov A. (2010). Efektyvna dezinfektsiia ta pidbir dezynfektanta [Effective disinfection and selection of disinfectant]. Zhurnal Veterynariia [Journal of Veterinary Medicine], 2, 18-22.(in Ukrainian)
15. Kolos Yu., Stets V., Tytarenko V. (2007). Rol sanitarnoi obrobky – dezinfektsii u pidtrymanni stabilnoho epizootychnoho blahopoluchchia u ptakhivnytstvi [The role of sanitation - disinfection while maintaining stable epizootic welfare in poultry]. Veterynarna medytsyna Ukrainy [Veterinary medicine of Ukraine], 12, 28-30. (in Ukrainian)
16. Kotsiumbas I., Malyk, O., & Patereha, I. (2006). Doklinichni doslidzhennia veterynarnykh likarskykh zasobiv [Preclinical studies of veterinary drugs]. Vydavnytstvo Triada plius Lviv [Triada plus Lviv publishing house], 360. (in Ukrainian)
17. Kotsiumbas I., Serhiienko O., Kovalchyk L., et al.. (2010). Suchasni zasoby veterynarnoi dezinfektsii [Modern means of veterinary disinfection]. Veterynarna medytsyna Ukrainy [Veterinary medicine of Ukraine], 1, 36-38. (in Ukrainian)
18. Kovalenko V.. (2012). Suchasni dezinfektanty na kontroli biobezpeky. [Modern disinfectants for biosafety control]. Biuleten «Veterynarna biotekhnolohiia» [Veterinary Biotechnology Bulletin], 21, 61–71. (in Ukrainian)