MILK PRODUCTIVITY ANALYSIS OF UKRAINIAN SELECTION CATTLE BREEDS WITH DIFFERENT GENOTYPES BY IFNGR2 LOCUS
Abstract
The study of genetic variability by quantitative traits loci and search for the associations of various allelic variants of genes with animal productivity traits in Ukrainian selection cattle breeds are important for identifying and preserving potentially valuable alleles and genotypes in the gene pool of Ukrainian cattle, as well as their possible use in marker-associated selection. Therefore, the aim of the work was to analyze the productive parameters of Ukrainian Black-and-White dairy breed cattle and Red-and-White dairy breed cattle with different genotypes by IFNGR2 locus (1008A>G). Genotyping of individuals was carried out using the PCR-RFLP method. To analyze the milk productivity of animals, were used the values of average milk yield for 305 days of lactation, protein content in milk and milk fat. The analysis of productive qualities was conducted by comparing the parameters of three lactations for each group of animals. By IFNGR2 polymorphism in Ukrainian Blackand- White dairy breed the maximum values of the 305-day milk yield for all three lactations are specific for homozygous individuals GG. In Ukrainian Red-and-White dairy breed cattle, the dominant values of the milk yield during all three lactations are observed for AG heterozygous. In case of Ukrainian Black-and-White dairy breed, the comparison of milk productivity parameters was carried out for all possible genotypes – AA, AG and GG, in case of Red-and-White dairy breed – only for two genotypes AA and AG. There were established significant differences between groups of individuals with different genotypes for the second lactation by the parameter of milk yield in both experimental cattle populations. There were no significant differences between groups of animals with different genotypes by IFNGR2 locus (1008A>G) for both experimental breeds of cows during all three lactations for the parameters of milk protein and fat content. By the values of protein content in milk for three lactations, the experimental groups of animals were characterized by a normal distribution according to the Shapiro-Wilk test, which made it possible to use parametric criteria to analyze significant differences between the parameters of individuals with different genotypes.
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