Preclinical studies of disinfective properties of «Kontavir»
Abstract
One of the unsolved problems for sanitation and hygiene in veterinary medicine is the emergence of resistance in microorganisms to certain groups of disinfectants that are constantly used. Therefore, there is a need to create new comprehensive disinfectants. The aim of the work was to conduct preclinical studies of the disinfectant properties of «Kontavir». The research was conducted in the educational and scientific laboratory «Veterinary Pharmacy» of Sumy National Agrarian University.
Bactericidal dilution and phenolic coefficient of «Kontavir» disinfectant were determined. The experiment began with the preparation of a solution of «Kontavir» disinfectant and cultures of microorganisms E. coli and S. aureus. It was found that the bactericidal action of «Kontavir» is 131,5 times stronger than the bactericidal action of Сarbolic Acid. In the presence of high molecular weight protein bactericidal action of «Kontavir» is reduced by 1,61 times.
The effectiveness of «Kontavir» disinfectant was determined on test objects: concrete, plastic, tile and metal. Determination of the quality of the disinfection was performed after 10, 40 and 60 minutes. It has been proven that the «Kontavir» disinfectant exhibits bactericidal properties after 10 minutes of exposure at a concentration of 0,25% on the surface of metal, plastic and tile. On an inhomogeneous concrete surface, the disinfectant destroys E. coli colonies after 60 minutes of exposure.
The bactericidal activity of «Kontavir» disinfectant was also studied by the suspension method against enterobacteria, gram-positive cocci, gram-negative rods and bacilli. The 0,1% concentration of «Kontavir» disinfectant exhibits bactericidal activity against S. aureus 209-P, Salmonela Cholerasuis, Streptococcus faecium, Clostridium perfringens, Klebsiella spp. at 60 minutes of exposure, and as regarding Enretobacter spp. at 30 minutes of exposure. In concentrations of 0,25 and 0,5% the disinfectant exhibits antimicrobial properties against S. aureus 209-P, Salmonela Cholerasuis, Streptococcus faecium, Clostridium perfringens, Klebsiella spp., Enretobacter spp. at 30 minutes of exposure.
Studies of the virucidal action of «Kontavir» by suspension method were performed against DNA and RNA-containing viruses. It was found that a 0,25% concentration of «Kontavir» at 30 minutes exposure has a virucidal effect on the causative agent of transmissible gastroenteritis of pigs; at 60 minutes exposure has a virucidal effect on Aujeszky's disease; parainfluenza-3 cattle and viral diarrhea of cattle. A 0,25% concentration of «Kontavir» at 60 minutes exposure has a virucidal effect on the pathogen of Teshen's disease; a 0,5% concentration at an exposure of 30 minutes has a virucidal effect on the pathogens of Newcastle's, Gamboro's and Marek's diseases.
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