Cord blood and the prospects for the use of its components in the practice of veterinary surgery.

Abstract

The article presents the results of studies of glycoproteins, glycosaminoglycans and ceruloplasmin in the blood plasma of cattle in the dynamics of healing of post-castration wounds using intraperitoneal injections of cord blood serum.

The aim of the study was to determine the effect of parenteral administration of cord blood serum on the metabolism of connective tissue in clinically healthy animals and in aseptic inflammation. The studies were conducted on gobies of Simmental breed which were castrated by the closed method according to the generally accepted method. Subsequently, the animals were divided into two groups: control and experimental. Immediately after castration, animals of the experimental group were intraperitoneally injected with 5 ml of cryopreserved homologous cord blood serum. Intraperitoneal injections of homologous cord blood serum to animals of the experimental group were performed once a day for five days (5 injections in total).

Cord blood for serum was taken from the umbilical cord vessels during physiological delivery in cows. After centrifugation, the serum was poured into 5 ml sterile plastic tubes and cryopreserved in a freezer at 18–20 ° C, which was stored for 3-4 weeks.

Animals of the control group were intraperitoneally injected with 5 ml of physiological sodium chloride solution as a placebo.

Blood for studies in animals of both groups was taken before castration, on the 5th and 11th day of research.

In the blood plasma, the content of markers of connective tissue metabolism was determined - hexoses of glycoproteins (G-GP) and glycosaminoglycans (G-GAG) by the fractional method according to I.V. Neverov and N.I. Titarenko (1979) in an orcinol reaction with fractionation with ethanol and cetylpyridinium chloride.

The level of ceruloplasmin was determined by the method based on the oxidation of n-phenylenediamine with the participation of this metalloprotein.

The resulting digital material was processed by the methods of variation statistics using the parametric student criterion.

Intraperitoneal injections of cord blood serum help maintain the optimal ratio between the level of glycosaminoglycans and glycoproteins in the blood plasma of operated animals under conditions of the maximum manifestation of the inflammatory reaction in castration wounds

It has been established that the use of intraperitoneal administration of cord blood serum allows limiting the destruction of the connective tissue matrix and enhances and modulates the acute phase response of the body to the development of the inflammatory response.