Keywords: calves, bacterial antagonism, antibiotic resistance, B. amyloliquefaciense, B. mucilaginosus, B. coagulans, B. megaterium, B. pumilus


Improper and uncontrolled use of antimicrobials in livestock can lead to increased antibiotic resistance and affect animal and human health. The research were conducted in the farm of Holstein cattle LLC AF “Lan” Ukraine in March-April 2021. We monitored microorganisms on the farm, determined their number and species. Endo agar was used as the elective medium for Escherichia; Staphylococcus aureus was determined on Chistovich's agar, and fungi and yeast were determined on Saburo's agar. Polymerase chain reaction was used to determine Mycoplasma spp.. Antagonistic properties of probiotic strains of Bacillus spp. by diffusion into agar wells were also determined. The size of growth inhibition zone around different strains was determined in mm: Bacillus amyloliquefaciense NR 59, Bacillus mucilaginosus ACH 82, Bacillus coagulans ALM86, Bacillus megaterium NCH 55, Bacillus pumilus LA 56 in a dilution of 1 × 109, CFU/g. Disks with the antibiotic cephalexin were used as control. An appropriate strain of probiotic microorganism was poured into each well of meatpeptone agar with the appropriate isolate. Then incubated for 24 hours at 37 ºC and determined the demarcation zone around each well. The main pathogens of dairy calves on the farm are identified: S. agalactiae (23 %), S. aureus (11 %), S. epidermidis (18 %), E. fecalis (10 %), E. coli (12 %), Mycoplasma spp. (7 %), fungi Candida (9 %) and associated microflora (10 %). Three probiotic strains of microorganisms were identified, to which microorganisms which were isolated in the indoor of calf showed the greatest sensitivity. It was found that Bacillus coagulans ALM 86 showed more antagonistic properties compared to the antibiotic against S. agalactiae - by 18.93%; Candida - by 29.16%; S. aureus - by 15.56%. Bacillus pumilus LA 56 strain inhibited the colony's growth of S. epidermidis by 20.49%; E. coli 28.78%; Candida - by 7.33% more than cephalexin. B. megaterium NCH 55 showed antimicrobial properties against S. aureus and E. fecalis identical to the antibiotic cephalexin. As a result of the conducted research probiotics which can become an alternative of antibiotics are defined. The prospect of further research in this direction is to determine the mechanism of action of probiotics Bacillus megaterium NCH 55, Bacillus coagulans ALM 86 and Bacillus pumilus LA 56 on pathogenic microorganisms and determine the therapeutic effect on animals.


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How to Cite
Shkromada, O. I., & Dudchenko, Y. A. (2021). STUDY OF ANTIMICROBIAL ACTIVITY OF PROBIOTIC STRAINS OF BACILLUS. Bulletin of Sumy National Agrarian University. The Series: Veterinary Medicine, (4 (55), 38-43.