DETERMINATION OF THE IMMUNOSTIMULATORY EFFECT OF THE DRUG “INCOMBIVIT”
Recently, much attention has been paid to the study of the immune system of animals. In recent years, with immunodeficiency of animals, pharmacological immunocorrection through the use of immunomodulators has become increasingly important. Strong selection in search of higher growth rates of broilers has led to such side effects as metabolic disorders, low reactivity of the immune system. Insufficient supply of nutrients can weaken the immune system. It is safer to increase the immune potential of animals is the use of vitamins, minerals and other adaptogens. The level of acquired immunity depends on many factors, especially at the time of vaccination or infection. Vaccines interact with the immune system, eliciting the same immune response as in natural infection in birds. There are various ways to deliver antigens to chickens for immunization. In recent years, with immunodeficiency in animals, pharmacological immunocorrection has become increasingly important through the use of immunomodulators of various natures, which can increase or decrease the level of immune response. The use of methods of stimulating immune function in animals in veterinary medicine is explained by the fact that in the conditions of industrial animal husbandry, animals often have immunodeficiency, as a result of which they are susceptible to various diseases. Immunomodulation is performed to increase the productivity of chickens, increase the body’s resistance to pathogens. For this purpose, we used the drug "Incombivit". It is a combination drug that contains fat – and water –soluble vitamins, trace elements and amino acids that normalize metabolism, increase overall resistance, improve productivity, safety and reproductive function of animals. The preparation contains only endogenously available biologically active substances that are a natural components of animal feed and are naturally present in animal tissues. Studies of the drug "Incombivit" were conducted in identical conditions of the control and experimental groups. The experimental drug was fed through the water supply system after filling the poultry house, and while vaccinating livestock. Laboratory tests of serum samples were performed. There was an increase in the number of B –, T – lymphocytes, globulins, hemoglobin, total serum protein and other indicators, indicating an enhanced process of antibody production after immunization.
2. Bhatt, P., Shukla, S. K., Wani, M. Y., Tiwari, R., & Dhama, K. (2013). Amelioration of chicken infectious anaemia virus induced immunosuppression by immunomodulator and haematinic supplementation in chicks. Veterinarski arhiv, 83, 639–652.
3. Bhuiyan, M. S., Amin, Z., Bakar, A. M., Saallah, S., Yusuf, N. H., Shaarani, S. M., & Siddiquee, S. (March 2021 г.). Factor Influences for Diagnosis and Vaccination of Avian Infectious Bronchitis Virus (Gammacoronavirus) in Chickens. Veterinary Sciences, 8, 47. doi:10.3390/vetsci8030047
4. Broom, L. J., & Kogut, M. H. (2019). Deciphering desirable immune responses from disease models with resistant and susceptible chickens. Poultry science, 98, 1634–1642.
5. Chen, H., Yan, F. F., Hu, J. Y., Wu, Y., Tucker, C. M., Green, A. R., & Cheng, H. W. (March 2017 г.). Immune Response of Laying Hens Exposed to 30 ppm Ammonia for 25 Weeks. International Journal of Poultry Science, 16, 139–146. doi:10.3923/ijps.2017.139.146
6. Demchuk, M. V. (2003). Vymohy do rozvytku zoohihiienichnoi nauky v Ukraini na mezhi tysiacholit. [Requirements for development of zoogyogenic science in Ukraine on the verge of millennia] Vet. med. Ukrainy, 35–36. [in Ukrainian]
7. Genovese, K. J., He, H., Swaggerty, C. L., & Kogut, M. H. (2013). The avian heterophil. Developmental & Comparative Immunology, 41, 334-340. doi:https://doi.org/10.1016/j.dci.2013.03.021
8. Hieke, A.-S. C., Hubert, S. M., & Athrey, G. (March 2019 г.). Circadian disruption and divergent microbiota acquisition under extended photoperiod regimens in chicken. PeerJ, 7, e6592. doi:10.7717/peerj.6592
9. Hofmann, T., Schmucker, S. S., Bessei, W., Grashorn, M., & Stefanski, V. (July 2020 г.). Impact of Housing Environment on the Immune System in Chickens: A Review. Animals, 10, 1138. doi:10.3390/ani10071138
10. Hofmann, T., Schmucker, S., Sommerfeld, V., Huber, K., Rodehutscord, M., & Stefanski, V. (January 2021 г.). Immunomodulatory Effects of Dietary Phosphorus and Calcium in Two Strains of Laying Hens. Animals, 11, 129. doi:10.3390/ani11010129
11. Hubert, S. M., Al-Ajeeli, M., Bailey, C. A., & Athrey, G. (December 2019 г.). The Role of Housing Environment and Dietary Protein Source on the Gut Microbiota of Chicken. Animals, 9, 1085. doi:10.3390/ani9121085
12. Ike, A. C., Ononugbo, C. M., Obi, O. J., Onu, C. J., Olovo, C. V., Muo, S. O., Omeke, O. P. (January 2021 г.). Towards Improved Use of Vaccination in the Control of Infectious Bronchitis and Newcastle Disease in Poultry: Understanding the Immunological Mechanisms. Vaccines, 9, 20. doi:10.3390/vaccines9010020
13. Kidd, M. T. (April 2004 г.). Nutritional modulation of immune function in broilers. Poultry Science, 83, 650–657. doi:10.1093/ps/83.4.650
14. Kim, D. K., Kim, C. H., Lamont, S. J., Keeler Jr, C. L., & Lillehoj, H. S. (2009). Gene expression profiles of two B-complex disparate, genetically inbred Fayoumi chicken lines that differ in susceptibility to Eimeria maxima. Poultry science, 88, 1565–1579.
15. Kogut, M. H. (April 2019 г.). The effect of microbiome modulation on the intestinal health of poultry. Animal Feed Science and Technology, 250, 32–40. doi:10.1016/j.anifeedsci.2018.10.008
16. Kosenko, M. V., & Liubenko, Ya. M. (2001). Imunolohichni preparaty u veterynarnii praktytsi. [Immunological preparations in veterinary practice] Vet. med. Ukr, 22–24. [in Ukrainian]
17. Kosenko, M., Kotsiumbas, I., & Kosenko, Yu. (2004). Kontrol vplyvu veterynarnykh likarskykh zasobiv na stan imunitetu tvaryn ta in. [Control of influence of veterinary medicine on the condition of animal health and others] Veterynarna medytsyna Ukrainy, 43. [in Ukrainian]
18. Kryshtoforova, B. V. (2000). Virohidni shliakhy mihratsii materynskykh imunohlobuliniv ta yikh vplyv na rozvytok plodiv i zhyttiezdatnist neonatalnykh teliat. [Probable migration routes of maternal immunoglobulins and their effect on fetal development and viability of neonatal calves] BV Kryshtoforova, 14–15. [in Ukrainian]
19. Mot, D., Timbermont, L., Haesebrouck, F., Ducatelle, R., & Immerseel, F. V. (July 2014 г.). Progress and problems in vaccination against necrotic enteritis in broiler chickens. Avian Pathology, 43, 290–300. doi:10.1080/03079457.2014.939942
20. Paradowska, M., Dunislawska, A., Siwek, M., & Slawinska, A. (March 2022 г.). Avian Cell Culture Models to Study Immunomodulatory Properties of Bioactive Products. Animals, 12, 670. doi:10.3390/ani12050670
21. Peredera, S. B., Shcherbakova, N. S., Peredera, Zh. O., & Malinovska, A. Yu. (2018). Imunna systema ta epizootychnyi protses. [Immune system and epizootic process] BBK 48 S 91, 60. [in Ukrainian]
22. Ricke, S. C., Lee, S. I., Kim, S. A., Park, S. H., & Shi, Z. (February 2020 г.). Prebiotics and the poultry gastrointestinal tract microbiome. Poultry Science, 99, 670–677. doi:10.1016/j.psj.2019.12.018
23. Rubio, L. A. (February 2019 г.). Possibilities of early life programming in broiler chickens via intestinal microbiota modulation. Poultry Science, 98, 695–706. doi:10.3382/ps/pey416
24. Sheikh, I. S., Bajwa, M. A., Rashid, N., Mustafa, M. Z., Tariq, M. M., Rafeeq, M., Ullah, A. (2020). Effects of Immune Modulators on the Immune Status of Broiler Chickens. Pakistan Journal of Zoology, 52. doi:10.17582/journal.pjz/20190519110533
25. Simon, K., Verwoolde, M. B., Zhang, J., Smidt, H., de Vries Reilingh, G., Kemp, B., & Lammers, A. (2016). Longterm effects of early life microbiota disturbance on adaptive immunity in laying hens. Poultry science, 95, 1543–1554. doi:10.3382/ps/pew088
26. Song, B., Tang, D., Yan, S., Fan, H., Li, G., Shahid, M. S., Guo, Y. (March 2021 г.). Effects of age on immune function in broiler chickens. Journal of Animal Science and Biotechnology, 12. doi:10.1186/s40104-021-00559-1
27. Sugiharto, S. (June 2016 г.). Role of nutraceuticals in gut health and growth performance of poultry. Journal of the Saudi Society of Agricultural Sciences, 15, 99–111. doi:10.1016/j.jssas.2014.06.001
28. Sundlof, S. F. (2014). Veterinary Drugs Residues: Veterinary Drugs – General. Veterinary Drugs Residues: Veterinary Drugs – General, 35-38. doi:10.1016/b978-0-12-378612-8.00248-1
29. Swiatkiewicz, S., Arczewska-Wlosek, A., & Jozefiak, D. (March 2014 г.). Immunomodulatory efficacy of yeast cell products in poultry: a current review. World\textquotesingles Poultry Science Journal, 70, 57–68. doi:10.1017/s0043933914000051
30. Vlizlo, V. V., & Vishchur, O. I. (2011). Novyi klas imunotropnykh ta adiuvantnykh preparativ dlia profilaktyky zakhvoriuvan u tvaryn. [A new class of immunotropic and adjuvant drugs for the prevention of animal diseases] Veterynarna medytsyna, 45–47. [in Ukrainian]
31. Zuidhof, M. J., Schneider, B. L., Carney, V. L., Korver, D. R., & Robinson, F. E. (December 2014 г.). Growth, efficiency, and yield of commercial broilers from 1957, 1978, and 2005. Poultry Science, 93, 2970–2982. doi:10.3382/ps.2014-04291