EFFICIENCY OF TANSY TINCTURE AGAINST ECTOPARASITES IN ANIMALS

Keywords: tansy infusion, ectoparasites, intensity of invasion, ctenocephalidosis, blood, fur coat

Abstract

Reliable protection of animals from parasitic insects is the key to their health. Bites of blood-sucking insects, lice, ticks, and fleas cause anxiety, irritation, inflammatory and allergic reactions, toxemia, and exhaustion. Nowadays, there is a significant range of modern high-quality veterinary drugs on the market that have insecticidal and acaricidal properties. These products are characterized by different spectra of action, protective effects, and toxicity to warm-blooded animals. Sometimes, the use of some insecticides is limited due to their harmful impact on animals and the environment. Therefore, our research is devoted to studying the therapeutic effectiveness of a plant-based product as an alternative method of fighting ectoparasites. The aim of the study was to investigate the effect of using tansy tincture ("Brovapharma", Ukraine, active substance – protoveratrine) against ectoparasites in various animal species (cattle, small cattle, dogs). The tincture was diluted with non-chlorinated water in an equivalent ratio (1:1) and applied to the animal’s hair coat. Re-treatment with the preparation was carried out after 8-10 days. During the research, the causative agent of Haematopinus eurysternus (II = 11,7 ± 5,4 ex.) was found in cattle, Linognathus ovillus (II = 5,4 ± 0,6 ex.), L. pedalis (II = 3,5 ± 0,4 ex.) in sheep, and L. caprae (II = 2,3 ± 0,1 ex.) in goats. The main species of fleas found in the research dogs was Ctenocephalides felis (II = 17,1 ± 2,07 ex.), and to a lesser extent C. canis (II = 1,8 ± 0,4 ex.). In some dogs, of the German Shepherd breed, fleas Spilopsyllus cuniculi (II = 6,0 ± 0,7 ex.) were also found. Two hours after applying the preparation to the animal's hair coat, no live parasites were found (EE = 100%). Hematological studies were carried out on clinically healthy and C. felis – infected dogs. Morphological blood studies in affected dogs indicate leukocytosis of varying degrees (up to 13,25 ± 0,33 G/l), expressed eosinophilia (up to 15,33±0,52%), lymphocytopenia (up to 9,27±0,36%), and an increase in the number of rodshaped neutrophils (up to 2,6±0,20%). The number of erythrocytes in the blood of infested dogs decreases (by 10,4%), as does the hemoglobin content (by 16,45 %).

References

1. Adler, P. H. (2019). World Blackflies (Diptera: Simulidae): A Comprehensive Revision of the Taxonomic and Geographical Inventory. Clemson: Clemson University Publishing, 139.
2. Ahn, K. S., Huh, S. E., Seol, S. W., Kim, H. J., Suh, K. H., & Shin, S. (2018). Ctenocephalides canis is the dominant flea species of dogs in the Republic of Korea. Parasites & Vectors, 11(1), 196. DOI: 10.1186/s13071-018-2769-9.
3. Alavanja, M. C., & Bonner, M. R. (2012). Occupational pesticide exposures and cancer risk: a review. Journal of Toxicology and Environmental Health, 15(4), 238–263. DOI:10.1080/10937404.2012.632358.
4. Allan, B. F., Keesing, F., & Ostfeld, R. S. (2003). Effect of forest fragmentation on Lyme disease risk. Conservation Biology, 17(1), 267–272. DOI:10.1046/j.1523-1739.2003. 01260.x.
5. Artemenko, L. P., Goncharenko, V. P., Bukalova, N. V., Bahur, T. I., Antipov, A. A., Ljasota, V. P., Lytvynenko, O. P., & Bilan, A. S. (2020). Ektoparazytozy domashnikh i produktyvnykh tvaryn ta zasoby zakhystu [Ectoparasitoses of domestic and productive animals and ways to defend]. Scientific Bulletin of Veterinary Medicine, 2, 65–76. DOI: 10.33245/2310-4902-2020-160-2-65-76 (in Ukrainian)
6. Belykh, I. P. (2020). Treatment and prevention of arachnoses, entomoses and nematodoses in small breeds dogs and cats with a drug based on moxidectin. IOP Conference Series: Earth and Environmental Science, 548. 042034.
7. Chouhan, A. S., Choudhary, K., & Prakash, P. (2022). A Research on Herbal Treatment against Ectoparasites in Cattle. Journal of Animal Research & Veterinary Science, 6(2), 0.39. DOI:10.24966/ARVS-3751/100039
8. Clark, N. J., Seddon, J. M., Šlapeta, J., & Wells, K. (2018). Parasite spread at the domestic animal – wildlife interface: anthropogenic habitat use, phylogeny and body mass drive risk of cat and dog flea (Ctenocephalides spp.) infestation in wild mammals. Parasites & Vectors, 11(1), 8. DOI: 10.1186/s13071- 017-2564-z.
9. Coles, T. B., & Dryden, M. W. (2014). Insecticide/acaricide resistance in fleas and ticks infesting dogs and cats. Parasites & Vectors, 7(1), 8. DOI: 10.1186/1756-3305-7-8
10. Davidović, V., Joksimović Todorović, M., Stojanović, B., & Relić, R. (2012). Plant usage in protecting the farm animal health. Biotechnology in Animal Husbandry, 28(1), 87–98. DOI: 10.2298/BAH1201087D
11. Davidović, V., Lazarević, M., Joksimović Todorović, M., Maksimović, Z., & Jovanović, M. (2010): The effect of the extract of rhizome and root of hellebore (Helleborus odorus W. et K.) on parameters of white blood count and degree of phagocytosis in Wistar rats. Acta Veterinaria, 60(5–6), 605–618. DOI : 10.2298/AVB1006605D
12. Ellse, L., & Wall, R. (2014). The use of essential oils in veterinary ectoparasite control: a review. Medical and Veterinary Entomology, 28(3), 233–243. DOI:10.1111/mve.12033
13. Gálvez, R., Musella, V., Descalzo, M. A., Montoya, A., Checa, R., Marino, V., Martín, O., Cringoli, G., Rinaldi, L., & Miró, G. (2017). Modelling the current distribution and predicted spread of the flea species Ctenocephalides felis infesting outdoor dogs in Spain. Parasites & Vectors, 10(1), 428. DOI: 10.1186/s13071-017-2357-4.
14. Hayes, B., Schnitzler, B., Wiseman S., & Snyder D. E. (2015). Veterinary Parasitology, 207, 99–106.
15. Jamshidi, S., Maazi, N., Ranjbar-Bahadori, S., Rezaei, M., Morakabsaz, P., & Hosseininejad, M. (2012). A survey of ectoparasite infestation in dogs in Tehran, Iran. Revista Brasileira de Parasitologia Veterinária, 21(3), 326–329. DOI: 10.1590/s1984-29612012000300030
16. Kruchynenko, O. V. (2020). Ektoparazyty sobak i kotiv (poshyrennia ta likuvannia) [Ectoparasites of dogs and cats (spreading and treatment)]. Bulletin of Poltava State Agrarian Academy, 3, 241–250. DOI: 10.31210/visnyk2020.03.28 (in Ukrainian)
17. Macchioni, F., Perrucci, S., Cecchi, F., Cioni, P. L., Morelli, I., & Pampiglione, S. (2004). Acaricidal activity of aqueous extracts of chamomile flowers, Marticaria chamomilla, against the mite Psoroptes caniculi. Medical and Veterinary Entomology, 18(2), 205–207. DOI: 10.1111/j.0269-283X.2004.00488.x.
18. Maior, M. C., & Dobrota, C. (2013). Natural compounds with important medical potential found in Helleborus sp. Central European Journal of Biology, 8, 272–285. DOI: 10.2478/s11535-013-0129-x
19. Maleki-Ravasan, N., Solhjouy-Fard, S., Beaucournu, J. C., Laudisoit, A., & Mostafavi, E. (2017). The Fleas (Siphonaptera) in Iran: Diversity, Host Range, and Medical Importance. PLoS Neglected Tropical Diseases, 11(1), e0005260. DOI: 10.1371/journal.pntd.0005260.
20. Mashkey, A. M., Yevtushenko, A. V., Dotsenko, K. A., & Sumakova, N. V. (2017). Intehrovana systema zakhystu zhuinykh tvaryn vid ektoparazytiv [Integrated security ruminant animals from ectoparasites]. Veterinary Medicine, 103, 396–399. (in Ukrainian)
21. Mayera, M., Vogl, C. R., Amorena, M., Hamburger, M., & Walkenhorst, M. (2014). Treatment of Organic Livestock with Medicinal Plants: A Systematic Review of European Ethnoveterinary Research. Forsch Komplementmed, 21, 375–386. DOI: 10.1159/000370216
22. Nueleanu, V-I. (2008). The effect of the unspecific therapy with hellebore (Helleborus purpurascens) on young sheep. Proceedings 43rd Croatian and 3rd International Symposium on Agriculture Opatija Croatia; 791:794.
23. Periyaveeturaman, C., Selvaraju, D., Kinhekar, A. S., Singh, P. K., & Ravikumar, R. (2015) Efficacy of herbal composition against ectoparasite infestation in dogs. Advances in Applied Science Research, 6, 242–245.
24. Pirali-Kheirabadi, K., & Razzaghi-Abyaneh, M. (2007). Biological activities of chamomile (Matricaria chamomile) flowers’ extract against the survival and egg laying of the cattle fever tick (Acari Ixodidae). Journal of Zhejiang University Science B, 8(9), 693–696. DOI: 10.1631/jzus.2007.B0693
25. Proskurina, I. V., & Nahorna, L. V. (2019). Bioloho-ekolohichna kharakterystyka zbudnykiv entomoziv velykoi rohatoi khudoby [Biological and ecological characteristics by agents of entomozes a cattle]. Scientific and Technical Bulletin of State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives and Institute of Animal Biology, 20(1), 139–144. (in Ukrainian)
26. Seyoum, Z., Tadesse, T., & Addisu, A. (2015). Ectoparasites Prevalence in Small Ruminants in and around Sekela, Amhara Regional State, Northwest Ethiopia. Journal of Veterinary Medicine, 2015. DOI: 10.1155/2015/216085
27. Shevchenko, А. М. (2013). Parazytozy velykoi rohatoi khudoby stiilovoho periodu ta suchasnyi stan rynku insektoakarytsydnykh preparativ Ukrainy [Parasitosis cattle stall period and the current state of the market insecto-acaricidal preparations in Ukraine]. Veterinary Medicine of Ukraine, 4(206), 15–18. (in Ukrainian)
28. Shevchenko, А. М. (2019). Shchodo kontroliu napadu zoofilnykh mukh na koriv v umovakh tvarynnytskykh prymishchen [Control of zoophilic flies’ attacking dairy cows in livestock premises]. Bulletin of Poltava State Agrarian Academy, 2, 232–237. DOI: 10.31210/visnyk2019.02.31 (in Ukrainian)
29. Xhaxhiu, D., Kusi, I., Rapti, D., Visser, M., Knaus, M., Lindner, T., & Rehbein, S. (2009). Ectoparasites of dogs and cats in Albania. Parasitology Research, 105(6), 1577–1587. DOI: 10.1007/s00436-009-1591-x.
Published
2023-06-06
How to Cite
Dovhii, Y. Y., Berezovskyi, A. V., Prus, P. M., Zghozinska, O. A., & Bezditko, L. V. (2023). EFFICIENCY OF TANSY TINCTURE AGAINST ECTOPARASITES IN ANIMALS. Bulletin of Sumy National Agrarian University. The Series: Veterinary Medicine, (1(60), 33-38. https://doi.org/10.32782/bsnau.vet.2023.1.6