THE EFFECT OF PROBIOTIC LACTOBACILLUS CASEI B-7280 ADDED IN DIFFERENT DOSES WITH SUGAR SYRUP ON THE VITALITY OF BEES
Abstract
The influence of the probiotic Lactobacillus casei B-7280 as part of stimulating feeding on the vital activity of honey bees was studied. Three groups of bees, 60-90 in each, were formed and selected from analogous families by weight, family strength, and queen age. The research was carried out under thermostat conditions. Bees of the control (C) group were fed with 60% sugar syrup in the amount of 2 ml/group/day. Experimental 1 group of bees (E 1) received the probiotic Lactobacillus casei B-7280 at a concentration of 109 CFU/ml with 2 ml of sugar syrup; experimental 2 group of bees (E 2) received probiotic Lactobacillus casei B-7280 at a concentration of 106 CFU/ml with 2 ml of sugar syrup. Bees were fed daily. The duration of feeding with syrup and probiotics is 30 days. The physiological state of the bees was recorded daily throughout the study period. Dead and alive bees were counted. Catalase activity was determined on a spectrophotometer using the ability of hydrogen peroxide to form a stable colored complex with molybdenum salts. The obtained results prove that the probiotic Lactobacillus casei B-7280 has a stimulating effect on the viability of honey bees of the Carpathian breed during 30 days of its use with sugar syrup under the conditions of a laboratory thermostat. Feeding the probiotic Lactobacillus casei B-7280 to honey bees in doses of 1×109 CFU/ml (E 1 group) and 1×106 CFU/ ml (E 2 group) under the conditions of a laboratory thermostat stimulated their viability, which is confirmed by the greater number of live bees in 6 -day periods of the experiment compared to the control group. The obtained results correlate with an increase in the catalase activity of bee body tissues and indicate a pronounced physiological effect of the applied doses of probiotic B-7280. Addition of probiotic B-7280 to sugar syrup increased (Р<0.05) the catalase activity of bee tissues both under the influence of higher (E 1) and lower (E 2) doses. The applied doses of the probiotic Lactobacillus casei B-7280 increased the viability of bees based on a number of live bees, which was more pronounced on 12, 24 and 30 days of the experiment. The average number of bees in the E 1 and E 2 groups for 30 days of the research exceeded the control group by 3.7 and 4.4%, respectively, but on the 24th and 30th days was higher by 8.6 and 12.3% in E 1 and 6.4 and 8.6% – in E 2 groups. These data indicate the advisability of using the probiotic Lactobacillus casei B-7280 in the applied concentrations to increase the viability of honey bees.
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