ДОСЛІДЖЕННЯ ВПЛИВУ ПРОБІОТИКУ НА МІКРОБІОЦЕНОЗ КИШЕЧНИКУ ПОРОСЯТ

O. I. Shkromada; R. V. Hrek

doi:10.32782/bsnau.vet.2023.3.16

O. I. Shkromada https://orcid.org/0000-0003-1751-7009
R. V. Hrek https://orcid.org/0000-0001-9662-5176

DOI: https://doi.org/10.32782/bsnau.vet.2023.3.16

Keywords: piglets, probiotics, intestinal microflora, Lactobacillus sp., Bifidobacterium sp.

Abstract

An important factor for piglets is the maintenance of immunity due to the influence on the intestinal microflora and nonspecific immunity. The most responsible period for piglets is immediately after birth, when the morphological structure of the intestines (crypt height) and microbiome are formed. The research was carried out at the State Enterprise "DG of the Institute of Agriculture of the Northeast" of the National Academy of Sciences of Ukraine in March 2022. The breed of pigs is Big White + Landrace. The amount of Lactobacillus sp. in the intestinal contents of piglets of the experimental groups there was more B. coagulans by 200.0%, B. mucilaginosus by 379.0%, B. megaterium by 228.7%, B. pumilus by 17.8%, with B. amyloliquefaciense – by 26.0% compared to the control. Bifidobacterium sp. in all experimental groups it was within 107, compared to the control 105. The number of Clostridium p. with B. coagulans was less by 93.3%, with B. mucilaginosus – by 66.6%, with B. megaterium, B. pumilus and B. amyloliquefaciense – by 33.3% in comparison with the control. Escherichia coli, which has hemolytic activity, was isolated from the feces of piglets with B. pumilus, B. amyloliquefaciense and control groups. The amount of Escherichia coli was isolated in a smaller amount in the first experimental group by 86.95%, the second by 88.0%, the third by 76%, the fourth by 65.2%, the fifth by 34.78%, compared with control Bacteria of the genera Salmonella and Pseudomonas were not isolated in the faecal masses of piglets. The number of yeast-like fungi in the samples of the experimental and control group of piglets was the same, less than 10 in 1cm3. Enterobacteriaceae were isolated from the piglets of the experimental groups by 98% less, compared to the control. Staphylococci were isolated in the first, second, fourth and fifth experimental groups in the same amount and 85.7% less, compared to the control. Research has established that the use of probiotic strains of Bacillus coagulans, Bacillus megaterium and Bacillus mucilaginosus had a positive effect on the content of beneficial microflora in the intestines of piglets and inhibited the growth of opportunistic microflora.

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STUDY OF THE INFLUENCE OF PROBIOTICS ON THE MICROBIOCENOSE OF THE INTESTINES OF PIGLETS

Abstract

References