DETERMINATION OF TOXIC PROPERTIES OF THE DRUG "COMBIOD"

Abstract

Fighting antibiotic resistance is a global priority today. Antibiotic resistance is largely due to the overuse of antibiotics and lack of awareness of the problem. Antibiotic resistance poses a huge threat to humanity. Given the widespread use of antibiotics in agriculture, there is a need to introduce and use environmentally friendly antimicrobial agents. The aim of this experiment was to investigate the toxicity of Combiod as an alternative antimicrobial agent. The aim of the work was to investigate the acute toxicity and harmlessness of the drug "Combiod". Materials of the experiment – red-brown solution with a faint odor of iodine, 1 ml of the drug contains: povidone-iodine – 200 mg, sodium selenite – 1.2 mg. The experiments were carried out in the certified vivarium of the Regional Center RC "ECOMEDCHEM" of Sumy State University and Sumy National Agrarian University. All experimental studies on laboratory animals were performed in accordance with Directive 2010/63/EU. The parameters of acute toxicity at a single administration were studied in 40 white mice weighing 19.0-20.3 g and 40 white rats weighing 185-205 g. Establishment of toxicity parameters for prolonged oral administration was performed on 18 rats weighing 190±5.0 g, divided equally into 3 groups, which were selected according to the principle of the previous groups. The effect of the drug on the state of internal organs was determined using the ratio of mass coefficients of viscera to body weight of the killed rats of the experimental and control groups. The skin-resorptive effect was determined on white mice weighing 21-24 g. The local irritant effect was determined by the method of cutaneous applications on 10 rabbits weighing 2.75±0.25 kg. To study the harmful effect on the ocular mucosa by the conjunctival test, 6 experimental groups of rabbits, 3 animals each, were used. When Combiod was administered orally at doses of 4,024, 50, 100, 500, 1500, 2500 and 5000 mg/kg body weight, no changes in the clinical condition of the experimental animals and no deaths occurred. With prolonged oral administration of the drug for 30 days at doses of 4.024 mg/kg and 2500 mg/kg body weight, no changes in the clinical status and behavioral reactions of rats were observed. The drug has no negative effect on the hematopoietic system. The working solution of the drug at a concentration of 0.002% does not cause allergic reactions. According to the results of the experiment, it was found that this drug, in accordance with the International Standard GOST 12.1.007-76, can be classified as class IV toxicity (low-toxic substances), and according to the Global Harmonized System (GHS), it is classified as category 5. The prospect of further research in this direction is to determine the effect of the drug "Сombiod" on the quality and safety of poultry products.