APPLICATION OF PROBIOTIC SUPPLEMENT FOR KETOSIS IN COWS
Abstract
The problem of treating ketosis often arises after calving, especially in high-yielding cows. The paper presents the results of the therapeutic effect of samples of Bacillus subtilis AX 20, Bacillus licheniformis EA 22 strains for ketosis in cows. The aim of the study was to determine the therapeutic dose of a probiotic supplement for lactating cows The experiment was conducted on a farm where Holstein cows are kept. Cows suffering from ketosis in the research groups were fed concentrated feed with the addition of Bacillus subtilis АХ 20, Bacillus licheniformis EA 22 (1×109 CFU/g) at the rate of 15-35 g per animal. The duration of the study was thirty days. Administration of 15 g of probiotic to cows reduced the level of ketones by 125% on the fifth day, by 100% on the fifteenth day, and by 42.85% on the 30th day, compared to the control. With the addition of 25 g of probiotics, the level of ketones decreased by 125% on the fifth day, by 114.28% on the fifteenth day, and by 28.57% on the 30th day. In the experimental group of cows with the addition of 35 g, the content of β-ketones decreased by 125% on the fifth day, by 71.42% on the fifteenth, and by 30, similar to the control. The level of β-ketones in the blood of the control group of animals corresponded to the physiological norm (1.0 mmol/l). Studies have proven that on the 30th day of the study, the level of ketone bodies in all animals corresponded to the physiological norm. The use of probiotics in a dose of 35 g to cows contributed to the maximum increase in the number of Lactobacillus sp. by 64%, Bifidobacterium by 58%, compared to the control group. Also, the content of Escherichia coli – by 45%, Clostridium – by 27%, Enterobacteriaceae and Staphylococcus – by 75%, Candida – by 80% decreased significantly. As a result of the application of probiotics to cows at a dose of 15 g in the group, on the fifteenth day of the research, kMAFAnM decreased by 14.48%, CSK – by 26.55%. On the thirtieth day of the experiment, kMAFAnM decreased by 40.45%, CSK – by 45.59%, compared to the beginning of the research. In the milk of cows with the use of probiotics in a dose of 25 g, on the fifteenth day the amount of kMAFAnM decreased by 21.2% and CSC by – 58.2%, on the thirtieth day kMAFAnM decreased by 40.51%, CSC – by 62.48%, compared to before the beginning of the study.
References
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