HEMATOLOGICAL PARAMETERS OF BROILER CHICKENS USING PROBIOTICS

Keywords: metabolism of chickens, hematological status, probiotic strain, nutrition of the diet

Abstract

The widespread use of antibiotics to stimulate growth and prevent bacterial infections in poultry has led to the emergence of resistant strains of bacteria. In addition, drug residues in meat directly or indirectly threaten human health and environmental safety. Against the background of this problem, there was a need to develop a new method of stimulating the growth of poultry and increasing its resistance. The aim of the work was to determine the effect of different concentrations of the probiotic B. coagulans on the hematological parameters of broiler chickens. The research was conducted in the conditions of the vivarium of the Faculty of Veterinary Medicine of the Sumy National Agrarian University in October 2023. Broiler chickens (cross Cobb- 500) were chosen as the object of research, from which 4 research groups were formulated, and one control group of 25 heads each. Research methods were used in the work: hematological, biochemical, physiological and statistical methods of blood serum research. In the experimental groups, an increase in the number of erythrocytes and leukocytes was recorded in the first by 45.54-4.82%, in the second by 58.41-18.63%, in the third by 101.48-25.44% (*Р<0, 05). In the blood serum of experimental groups of chickens, the level of platelets in the first group decreased by 15.85%, in the second group by 21.54%, and in the third group by 34.14%. In the chickens of the third experimental group, the level of cholesterol decreased by 24.77%, triglycerides by 54.80% (*Р<0.05), urea by 34.13%, and creatinine by 21.69%. The content of total protein and globulin in the chickens of the third experimental group was probably higher by 56.27 and 70.99%, respectively (*Р<0.05), compared to the control groups. The activity of the enzymes aspartate aminotransferase and alanine aminotransferase was similar in the experimental and control groups, which proves the absence of toxic effects of the probiotic B. coagulans in different concentrations (1×105, CFU/g, 1×107, CFU/g, 1×109, CFU/g) on internal organs and tissues. The practical value of the study lies in the prevention of dysbacteriosis and immunodeficiency in broiler chickens. The perspective of further research in this direction is to determine the influence of B. coagulans on carcass properties and meat quality.

References

1. Bai, K., Huang, Q., Zhang, J., He, J., Zhang, L., & Wang, T. (2017). Supplemental effects of probiotic Bacillus subtilis fmbJ on growth performance, antioxidant capacity, and meat quality of broiler chickens. Poultry science, 96(1), 74–82. https://doi.org/10.3382/ps/pew246
2. Biswas, A., Dev, K., Tyagi, P. K., & Mandal, A. (2022). The effect of multi-strain probiotics as feed additives on performance, immunity, expression of nutrient transporter genes and gut morphometry in broiler chickens. Animal bioscience, 35(1), 64–74. https://doi.org/10.5713/ab.20.0749
3. Ferdous, F., Saski, C., Bridges, W., Burns, M., Dunn, H., Elliott, K., & Scott, T. R. (2016). Transcriptome Profile of the Chicken Thrombocyte: New Implications as an Advanced Immune Effector Cell. PloS one, 11(10), e0163890. https://doi.org/10.1371/journal.pone.0163890
4. Fotina, T. I., & Sergeychik, T. V. (2022). MONITORING OF RISK FACTORS ON FARMS TO KEEP CHICKEN BROILERS. Bulletin of Sumy National Agrarian University. The Series: Veterinary Medicine, (1 (56), 31-36. https://doi.org/10.32845/bsnau.vet.2022.1.5
5. Gibson, G.R., Hutkins, R., Sanders, M.E., Prescott, S.L., Reimer, R.A., Salminen, S.J., Scott, K., Stanton, C., Swanson, K.S., Cani, P.D., Verbeke, K., & Reid, G. (2017). Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nature Reviews Gastroenterology & Hepatology, 14, 491–502. doi: 10.1038/nrgastro.2017.75.
6. Grant, A., Gay, C.G., & Lillehoj, H.S. (2018). Bacillus spp. as direct-fed microbial antibiotic alternatives to enhance growth, immunity, and gut health in poultry. Avian Pathology, 47(4), 339–351. doi: 10.1080/03079457.2018.1464117.
7. Johnson, T.J., Youmans, B.P., Noll, S., Cardona, C., Evans, N.P., Karnezos, T.P., Ngunjiri, J.M., Abundo, M.C., & Lee, C.W. (2018). A consistent and predictable commercial broiler chicken bacterial microbiota in antibiotic-free production displays strong correlations with performance. Applied and Environmental Microbiology, 84(12), article number 00362-18. doi: 10.1128/AEM.00362-18.
8. Liu, Z., Jiang, Z., Zhang, Z., Liu, T., Fan, Y., Liu, T., & Peng, N. (2022). Bacillus coagulans in combination with chitooligosaccharides regulates gut microbiota and ameliorates the dss-induced colitis in mice. Microbiology Spectrum, 10(4), article number 0064122. doi: 10.1128/spectrum.00641-22.
9. Ngunjiri, J.M., Taylor, K.J.M., Abundo, M.C., Jang, H., Elaish, M., Kc, M., Ghorbani, A., Wijeratne, S., Weber, B.P., Johnson, T.J., & Lee, C.W. (2019). Farm stage, bird age, and body site dominantly affect the quantity, taxonomic composition, and dynamics of respiratory and gut microbiota of commercial layer chickens. Applied and Environmental Microbiology, 85(9), article number 03137-18. doi: 10.1128/AEM.03137-18.
10. Sugiharto, S. (2016). Role of nutraceuticals in gut health and growth performance of poultry. Journal of the Saudi Society of Agricultural Sciences, 15(2), 99-111. https://doi.org/10.1016/j.jssas.2014.06.001
11. Sugiharto, S., Yudiarti, T., Isroli, I., Widiastuti, E., & Wahyuni, H. I. (2018). Hematological parameters and selected intestinal microbiota populations in the Indonesian indigenous crossbred chickens fed basal diet supplemented with multi-strain probiotic preparation in combination with vitamins and minerals. Veterinary world, 11(6), 874–882. https://doi.org/10.14202/vetworld.2018.874-882
12. Tang, X., Liu, X., & Liu, H. (2021). Effects of Dietary Probiotic (Bacillus subtilis) Supplementation on Carcass Traits, Meat Quality, Amino Acid, and Fatty Acid Profile of Broiler Chickens. Frontiers in veterinary science, 8, 767802. https://doi.org/10.3389/fvets.2021.767802
13. Wu, Y., Shao, Y., Song, B., Zhen, W., Wang, Z., Guo, Y., Shahid, M.S., & Nie, W. (2018). Effects of Bacillus coagulans supplementation on the growth performance and gut health of broiler chickens with Clostridium perfringens-induced necrotic enteritis. Journal of Animal Science and Biotechnology, 9, article number 9. doi: 10.1186/s40104-017-0220-2.
14. Yang, J., Qian, K., Zhang, W., Xu, Y., & Wu, Y. (2016). Effects of chromium-enriched bacillus subtilis KT260179 supplementation on chicken growth performance, plasma lipid parameters, tissue chromium levels, cecal bacterial composition and breast meat quality. Lipids in health and disease, 15(1), 188. https://doi.org/10.1186/s12944-016-0355-8
15. Zheng, W., Zhao, Z., Yang, Y., Ding, L., & Yao, W. (2023). The synbiotic mixture of lactulose and Bacillus coagulans protects intestinal barrier dysfunction and apoptosis in weaned piglets challenged with lipopolysaccharide. Journal of Animal Science and Biotechnology, 14, article number 80. doi: 10.1186/s40104-023-00882-9.
Published
2024-10-02
How to Cite
Fotina, T. I., & Sergeychik, T. V. (2024). HEMATOLOGICAL PARAMETERS OF BROILER CHICKENS USING PROBIOTICS. Bulletin of Sumy National Agrarian University. The Series: Veterinary Medicine, (2(65), 35-39. https://doi.org/10.32782/bsnau.vet.2024.2.6