ВИЗНАЧЕННЯ ПАРАМЕТРІВ ТОКСИЧНОСТІ ВЕТЕРИНАРНОГО ЗАСОБУ «АСПІР-35»

T. I. Fotina; G.U. Gavriluk

doi:10.32782/bsnau.vet.2024.4.15

T. I. Fotina Sumy National Agrarian University https://orcid.org/0000-0001-5079-2390
G.U. Gavriluk Sumy National Agrarian University https://orcid.org/0009-0004-7759-799X

DOI: https://doi.org/10.32782/bsnau.vet.2024.4.15

Keywords:

Abstract

The impact of heat stress is a serious problem that causes economic losses for poultry farming. Heat stress has a detrimental effect on physiological responses, such as immunity, oxidative stress, intestinal and muscle functions. Heat stress affects feed intake, antioxidant system, mitochondrial function and heat shock protein expression; disrupts the free radical homeostasis of the body and reorganizes the use of protein, fat and energy; this subsequently affects the productivity, reproduction and health of animals. The aim of the work is to conduct a toxicological assessment of the veterinary drug "Aspire-35" produced by the NPF "Brovapharma" (Ukraine). The following methods were used in the work: statistical, physiological state studies, pathoanatomical, histological, hematological. The article presents the results of studying the toxicity of the veterinary drug "Aspire-35" on laboratory animals. The drug "Aspire-35" at a single oral administration in doses of 1250, 2500 and 5000 mg/kg of body weight did not cause death of experimental rats and mice. Based on the conducted studies, it can be concluded that the maximum dose of the drug "Aspire-35", which does not cause death of experimental rats and mice at a single oral administration (LD0) is greater than the dose of 5000 mg/kg of body weight. On this basis, the drug "Aspire-35" can be attributed to the 4th hazard class according to the International Standard GOST 12.1.007-76, or to category 5 according to the International Global Harmonized System (GHS), since the LD50 of the drug "Aspire-35" at oral administration will exceed 5000 mg/kg of body weight. The drug "Aspire-35" at a dose of 0.5 ml/kg of body weight (twenty-five times the maximum therapeutic dose prescribed for the treatment of young cattle) when administered subcutaneously for 18 days did not cause any negative or harmful effects on the body of experimental rats, did not affect their growth and development, did not cause changes in the relative mass of internal organs and did not lead to changes in hematological indicators in experimental animals. When the animals were necropsied, no visible pathological changes were observed in the internal organs and tissues of rats. Also, no significant changes were recorded in the relative mass coefficients of the internal organs of rats to the body weight of rats at the end of the study. Further studies will be the next stage of pre-registration trials aimed at studying the toxicity of the drug "Aspire-35" upon repeated administration to birds (subacute toxicity upon oral administration), which is a mandatory material in the section "Safety and Residue Studies" of the dossier for this medicinal product.

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