EPIZOOTOLOGICAL FEATURES, DIAGNOSTICS AND EFFECTIVENESS OF TREATMENT OF CARNIVORES SICK OF DIROFILARIOSIS

Keywords: heartworm disease in dogs, causative agents, antiparasitic drugs

Abstract

The article presents the results of scientific research on epizootological features, diagnosis and effectiveness of treatment of carnivores with heartworm disease. Dirofilariasis is an infectious parasitic disease characterized by heart, liver and kidney problems. The disease affects dogs, cats, wild animals, and sometimes people. The causative agent of this disease is the nematodes Dirofilaria immitis, Dirofilaria repens and others. They have a transparent body covered with a transparent membrane. Large helminths 25-30 cm long, live, larvae (microfilariae) 0.22-0.29 mm long, with a diameter equal to the size of an erythrocyte. Dirofilaria immitis affects the right ventricle of the heart, the pulmonary artery, causing severe attacks of the pulmonary vein, right ventricle and right atrium. Dirofilaria is usually found in unusual places for parasites: in the eyes, brain, stomach, subcutaneous space, spine. Larvae enter the blood of the host through the insect's proboscis and develop in the animal's body. The larva's life span lasts about 7-8 months. One heartworm produces 30,000 larvae per day. Microfilariae circulate in the blood of pregnant women for three years. The maximum number of microdirofilariae in the peripheral blood of animals in the morning and in the evening, which corresponds to two peaks in the central nervous system. Direct microscopy of a drop of freshly collected blood from a carnivore at low magnification is the simplest, most convenient and fastest method of diagnosing heartworm disease. We also recommend using the research method according to Knott, according to Kulikov. It is also necessary to perform an autopsy of the chest cavity and examine the pericardium and other tissues for the presence of helminths. Prevention of heartworm infection in animals is primarily based on interrupting the transmissible transmission of infestation and consists of several directions: extermination of mosquitoes, detection and deworming of infested domestic dogs, prevention of contact of mosquitoes with domestic animals. Monthly treatment of animals against insects, good quality feeding, timely vaccination and deworming of animals are necessary [19, 21]. The drugs "Ivermectin", "Milbemycin" and "Dironet 500" have a prolonged effect. The use of these drugs with an interval of 30-35 days has a detrimental effect on microfilariae, which prevents the infection of other carnivores and prevents the spread of heartworm disease.

References

1. Adolph, C., Barnett, S., Beall, M., Drake, J., Elsemore, D., Thomas, J., & Little, S. (2017). Diagnostic strategies to reveal covert infections with intestinal helminths in dogs. Veterinary Parasitology, 247, 108–112. doi:10.1016/j.vetpar.2017.10.002.
2. Arbune, M., & Dobre, M. (2015). Dirofilariasis – an emergent human parasitosis in Romania. Acta Parasitologica, 60, 485–487. doi: 10.1515/ap-2015-0068.
3. American Heartworm Society. Canine guidelines. (2020). URL: https://d3ft8sckhnqim2.cloudfront.net/images/pdf/2020_AHS_Canine_Guidelines_Summary_11_12.pdf?1605556516
4. Genchi, C., Kramer, L. (2017). Subcutaneous dirofilariosis (Dirofilaria repens): an infection spreading throughout the old world. Parasit Vectors., 10(2):517-521. URL: https://d3ft8sckhnqim2.cloudfront.net/images/pdf/AHS_Canine_Guidelines_11_13_20.pdf?1605556516
5. Simon, F., Siles, M., Lucas, R., Morchon, J., Gonzalez-Miguel, I., Mellado, Carreton, E., Montoya-Alonso, J. A. (2012). Human and animal dirofilariasis: the emergence of a zoonotic mosaic. Clin Microbiol Rev., 25:507-544.
6. Panarese, R., Maria, R. I., Latrofa S., Zatell A., Ignjatović Ćupina, A., Montarsi, F., Pombi, M., Mendoza-Roldan, J. A., Beugnet, F., Otranto, D. (2020). Hyperendemic Dirofilaria immitis infection in a sheltered dog population: an expanding threat in the Mediterranean region. International Journal for Parasitology., 50(8):555-559. DOI: https://doi.org/10.1016/j.ijpara.2020.04.002
7. Knott, J. (1939). A method for making microfilarial surveys on day blood. Trans. Roy. Soc. Trop. Med. Hyg., 33:191-196.
8. Mikola, N., Oborina, V., Jokelainen, P. (2020). Knowledge about emerging zoonotic vector-borne parasites Dirofilaria immitis and Dirofilaria repens in Finland: questionnaire survey to medical doctors and veterinarians. Vector Borne Zoonotic Dis., 20:27- 32. DOI: https://doi.org/10.1089/vbz.2019.2502
9. Moorhead, A.R., Evans, C.C., Kaplan, R.M. 2017. A diagnostic algorithm for evaluating cases of potential macrocyclic lactone–resistant heartworm. Parasit Vectors., 10 (2): 479.
10. Ponomarenko, V.Ya., Fedorova, O.V., Bulavina, V.S. (2009). Parazytozy bezprytulnykh sobak – nebezpeka dlia zdorovia liudyny [Parasites of stray dogs are a danger to human health]. Veterinary medicine of Ukraine. Kyiv, 12. 14-17 [in Ukrainian].
11. Pupić-Bakrač, A., Pupić-Bakrač, J., Beck, A., Jurković, D., Polkinghorne, A., Beck, R., Pupić-Bakrač, A., et al. (2021). Dirofilaria repens microfilaremia in humans: Case description and literature review. One Health. 12; 13.doi: 10.1016/j. onehlt.2021.100306..
12. Atsumi, E., Matsumoto, H., Taira, N., Yohena, T., Kawasaki H., Kawabata, T., Yoshimi, N. (2019). Thirteen cases of pulmonary dirofilariоsis in a single institution in Okinawa Island. Virchows Archiv. 475: 335-340., DOI: https://doi.org/10.1007/ s00428- 019-02614-9
13. Yoshikawa, S., Ichikawa, Y., Nakagaki K., Matsumoto, J., Nogami, S. (2014). Prevalence of Dirofilaria immitis among shelter dogs in Tokyo, Japan, after a decade: comparison of 1999-2001 and 2009-2011. Parasite. 21:10., DOI: https://doi.org/10.1051/parasite/2014008
14. Ashley, B., Saunders, D., Wesselowski, S., Cusack, K. (2020). Transesophageal Echocardiography–Guided Dirofilaria immitis Extraction from the Right Atrium in a Dog. CASE. 4(4):299-302,. DOI: https://doi.org/10.1016/j.case.2020.05.005
15. Napoli, E., Bono, V., Gaglio, G., Giannetto, S., Zanghì, A., Otranto, D., Brianti., E. (2019). Unusual localization of Dirofilaria repens (Spirurida: Onchocercidae) infection in the testicle of a dog Comparative Immunology, Microbiology and Infectious Diseases., 66 :101326. DOI: https://doi.org/10.1016/j.cimid.2019.06.007
16. Nelson, C.T., McCall, J.W., Jones S., Moorhead, A. (2020). Current Canine Guidelines for the Prevention, Diagnosis and Management of Heartworm Infections in Dogs. URL: https://d3ft8sckhnqim2.cloudfront.net/images/pdf/2020_AHS_Canine_Guidelines.pdf?1580934824
17. FDA. (2020). Keep the Worms Out of Your Pet’s Heart! The Facts about Heartworm Disease. Available from: DOI: https://www.fda.gov/ animal-veterinary/animal-health-literacy/keep-worms-out-yourpets-heart-facts-about-heartwormdisease 18. Jiang, S., Tsikolia, M., Benner, U., Bloomquist, J. (2017). Mosquitocidal activity and mode of action of the isoxazoline fluralaner. Int J Environ Res Public Health., 14:154. Doi: 10.3390/ijerph14020154.
19. McCall, J.W., Kramer, L., Genchi, C., Guerrero, J., Dzimianski, M.T., Mansour, A., McCall, S. D., Carson, B. (2014). Effects of doxycycline on heartworm embryogenesis, transmission, circulating microfilaria, and adult worms in microfilaremic dogs. Vet Parasitol., 206(1-2):5-13.
20. McCall, J.W., Varloud, M., Hodgkins, E., Mansour A., DiCosty, U., McCall, S., Carmichael, J., Carson, B., Carter, J. (2017). Shifting the paradigm in Dirofilaria immitis prevention: blocking transmission from mosquitoes to dogs using repellents/insecticides and macrocyclic lactone prevention as part of a multimodal approach. Parasit Vectors., 10(2):525.
21. Savadelis, M.D., Day, K.M., Bradner, J.L., Wolstenholme, A.J., Dzimianski, M.T., Moorhead, A, R. (2018). Efficacy and side effects of doxycycline versus minocycline in the three dose melarsomine canine adulticidal heartworm treatment protocol. Parasit Vectors., 11:671.
22. Szczepanek-Parulska, E., Kludkowska, M., Pielok, L., Stefaniak, J., & Ruchala, M. (2017). Subcutaneous lesion in an oncologic patient. American Journal of Medicine, 130, 279–281. doi:10.1016/j.amjmed.2017.03.019.
23. Tappe, D., Plauth, M., Bauer, T, Muntau, B., Diebel, L., Tannich, E., et al. (2014). A case of autochthonous human Dirofilaria infection. Euro Surveillance, 19, 2–4. doi: 10.2807/1560- 7917.ES2014.19.17.20790.
24. Trancoso, T.A.L., Lima, N.C., Barbosa, A.S., Leles, D., Fonseca, A.B.M., Labarthe, N.V., Bastos, O.M.P., & Uchôa, C.M.A. (2020). Detection of Dirofilaria immitis using microscopic, serological and molecular techniques among dogs in Cabo Frio, RJ, Brazil. Braz. J. Vet. Parasitol; 29(1): e017219. doi:10.1590/S1984-29612020009.
25. Vlizlo, V.V., Fedoruk, R.S., Ratych, I.B. (2012). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni [Laboratory research methods in biology, animal husbandry and veterinary medicine], Lviv, 764. (in Ukrainian).
Published
2023-06-06
How to Cite
Risovanyi, V. I., Panasenko, O. S., Nazarenko, S. M., & Stepushenko, I. V. (2023). EPIZOOTOLOGICAL FEATURES, DIAGNOSTICS AND EFFECTIVENESS OF TREATMENT OF CARNIVORES SICK OF DIROFILARIOSIS. Bulletin of Sumy National Agrarian University. The Series: Veterinary Medicine, (1(60), 87-93. https://doi.org/10.32782/bsnau.vet.2023.1.14