STUDY OF THE TOXIC PROPERTIES OF THE NEW DRUG AGAINST ECTOPARASITOSIS OF POND FISH
Abstract
The article provides calculations of the acute toxicity of a new drug for the treatment of pond fish from ectoparasites based on povidone iodine. Parasitic diseases occupy a large part in the structure of diseases of infectious etiology of pond fish and can be a potential danger for consumers of aquaculture products. The most frequently recorded diseases are caused by protozoa, monogenia, cestodes and crustaceans. The development of the latest means for the treatment of fish from ectoparasites is a priority task of veterinary medicine. Compounds based on iodine povidone have proven themselves well in various fields of agriculture and medicine as a powerful antimicrobial, antiviral, fungicidal, antiprotozoal, disinfectant. Therefore, the development of a product based on povidone iodine in fish farming is promising. An important factor in the creation of a new medicinal product is the study of acute toxicity, and in this case the studies were conducted on fish, as the drug is planned to be used in aquaculture. The research was conducted on the basis of the Department of Veterinary Expertise, Microbiology, Zoohygiene and Safety and Quality of Livestock Products of the Faculty of Veterinary Medicine of the Sumy National Agrarian University. Five dilutions were used to determine the LD50 dilution of the water extract. Povidone iodine was given in the amount of 3000, 4000, 5000, 6000, 7000 mg/kg. Drinking water was used for the experiment. The pH value of the water was 7.6. Individuals of carp with an average body weight of 42±3 g were used to determine the parameters of acute toxicity. The research was carried out on carp using the methods of Kerber, Pershin and the computer program "LD50". As a result of the research, the following results of the calculation of the average lethal dose were obtained: 6800 mg/ kg according to the Kerber method, 6916.8 mg/kg according to the Pershin method, and 6925.9 mg/kg using the "LD50" computer program. As a result of the calculation of the average indicator, an indicator of 6880.9 mg/kg was established. According to GOST 12.1.007-76, the drug based on GOST 12.1.007-76 belongs to the 4th group of toxicity (low-toxic substances), since the dose of the drug that causes the death of fish is more than 5000 mg/kg.
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