ВИЗНАЧЕННЯ ГЕМАТОЛОГІЧНИХ ТА БІОХІМІЧНИХ ПАРАМЕТРІВ КРОВІ У КРОЛІВ, ХВОРИХ НА ПАСТЕРЕЛЬОЗ

V. A. Levitska

doi:10.32782/bsnau.vet.2024.3.4

V. A. Levitska Podilskyi State University https://orcid.org/0000-0003-3100-009X

DOI: https://doi.org/10.32782/bsnau.vet.2024.3.4

Keywords: pasteurellosis, hemorrhagic septicemia, phagocytic activity, inflammatory cytokines, lymphopenia.

Abstract

Pasteurellosis is a disease of rabbits characterized by rhinitis, pneumonia, orchitis, otitis media, septicemia and abscess formation. Sometimes P. multocida infection can be asymptomatic. Hematological and biochemical analysis of blood serum, analysis of inflammatory cytokines, immunological and histopathological analyzes were performed. The results showed that rabbits infected with P. multocida type B had macrocytic hypochromic anemia and leukocytosis with a significant increase in the percentage and index of phagocytic activity. Thus, the number of erythrocytes increased by 19.51%, hemoglobin concentration by 24.42%, in rabbits infected with P. multocida, compared to the control. In addition, an increase (p <0.05) of leukocytes, neutrophils by 160.30% and monocytes by 88.46% was observed. A decrease (p <0.05) in the number of lymphocytes by 32.71% and minor changes in the number of eosinophils by 38.46% were noted. Increase of phagocytic activity by 26.79% and phagocytic index by 55.88% in sick rabbits. Infected rabbits showed a significant decrease in total protein, albumin, globulin and immunoglobulin (IgG and IgM) serum levels. Thus, the content of total protein was lower in sick rabbits by 36.28%. At the same time, the amount of albumin was lower by 37.34%, and globulins by 31.97%, compared to healthy rabbits. The level of serum immunoglobulins showed a significant decrease (p <0.05) of IgG by 17.86% and IgM by 46.92%, compared to sick rabbits. The content of the inflammatory cytokine – interleukin was higher by 44.57% in the blood serum of patients with rabbit pasteurellosis. In addition, a significant increase in serum levels of inflammatory cytokines, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, and bilirubin (total, direct, and indirect) was observed in sick rabbits. Serum alanine transferase (ALT), alkaline phosphatase (AL) and lactate dehydrogenase (LDH) activities were significantly increased (p < 0.05) in the P. multocida-infected group compared to controls. Thus, the level of ALT increased by 40.93%, LF – by 87.78%, LDH – by 79.99% was higher in rabbits with pasteurellosis.

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DETERMINATION OF HEMATOLOGICAL AND BIOCHEMICAL PARАMETERS OF BLOOD IN RABBITS SICK OF PASTEURELLOSIS

Abstract

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