MICROELEMENTS OF BEE TISSUES FOR FEEDING WITH NANOTECHNOLOGICAL COBALT AND GERMANIUM CITRATES
Abstract
We examined the effect of nanotechnological citrates of Cobalt (30μg/300 ml), sugar syrup (s.c.) and Germanium (60μg/300 ml s.c.), and their mixture in aqueous solution on the content of individual elements in tissues of various anatomical regions and the whole body of the Carpathian bee. Four bee-analog groups were formed, control group (I) 300 ml s.c. per bee family/week, study group II (30 μg Coe/300 ml s.c.), study group III (60 μg Ge/300 ml s.c.), and study group IV (30 μg Coe + 60 μt Ge/300 ml s.c.). Bees were kept in gardens under 30 degrees in a laboratory thermostat for 30 days determining the content of Fe, Co, Ni, Cu, Pb i Cd in homogenates of head, breast, abdomen and whole body tissues. The microelements study was carried out using atomic absorption spectrophotometer SF-115 PC, in dry mineralizate of tissue homogenate. It was found probably the highest Ge content in the tissues of the thoracic section because of the effect of 60 μg Germanium and a citrate mixture with 30 μg Co and 60 μg Ge, against the background of the highest level of Cobalt in the tissues of all three study groups, but the lowest Lead for the tendency to decrease Cadmium and Nickel. The content of Fe, Ni, Pb i Cd, and Cu was reduced in the head tissues of bees of the study groups, only in the group IV there was an unlikely increase in Cobalt. There were established changes both in tissues of the head, abdomen and the whole organism of bees of the study groups compared to the control group, indicating certain physiological features of cumulation and distribution of the studied microelements in tissues and organs of bees during feeding with Cobalt and Germanium nanotechnology citrates. Of importance is the pronounced detoxifying effect of nanotechnological Cobalt and Germanium citrates and their combination, which provides a reduction in the concentration of Lead, Cadmium and Nickel in the tissues of both individual anatomical regions of bees and the whole organism. The antagonistic and synergistic dependence of the studied microelements in physiological processes and content in tissues of the body with other mineral elements of bee nutrition is discussed.
References
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18. Kykish, I. B., Koval'chuk, I. I. & Romaniv L. I. (2017). Vlijanie raznyh doz mikrojelementnogo preparata na vyzhivaemost' medonosnyh pchel [Influence of different doses of a microelement preparation on the survival of honey bees]. Sovremennye problemy veterinarnoj patologii i biotehnologii v agropromyshlennom komplekse: materialy Mezhdunarodnoj nauchno-prakticheskoj konferencii, posvjashhennoj 95–letiju RUP «Institut jeksperemental'noj veterinarii imeni S. N. Vyshelesskogo», Minsk, 16-17 November, 321–324 [in Russian]
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20. Lu, S. L., Tang, J. X. & Deng, L. S. (1997). Effects of Germanium Citrate on the Immunogenic Function of Mice. Guangdong Trace Elements Science, 4, 21–23.
21. Lukevics, E. & Ignatovich, L. (2003). Biological Activity of Organogermanium Compounds. Cheminform, 34, 279–295.
22. Malavolta, M., & Mocchegiani, E. (2018). Trace elements and minerals in Health and Longevity. Part of the Healthy Ageing and Longevity book series (series ed. Suresh I. S. Rattan), 8, 328.
23. Matuszewska, E., Klupczynska, A., Maciotek, K. Kokot, Z. I., & Matysiak J. (2021). Multielemental Analysis of Bee Pollen, Propolis, and Royal Jelly Collected in West-Central Poland. Molecules, 26(9), 2415. doi.org/10.3390/molecules26092415
24. Nefodova, O. O., Halperin, O. I., & Shatorna, V. F. (2019). The influence of cerium and germanium citrates on the process of embryogenesis of rat on the background of cadmium intoxication. Bulletin of problems biology and medicine, 1(1), (148), 273–278. doi:10.29254/2077-4214-2019-1-1-148-273-278.
25. Niu, Z. Y., Liu, F. Z., Wan, Y. S., Liang, X. & Xie, M. (2001). Effects of Germanium on the Growth of Main Immune Organs in Broilers. Journal of Northwest A & F University (Natural Science Edition), 10, 41–43.
26. Pashajan, A. S., Stolbov, N. M. (2008). Kormovye dobavki dlja pchel. Pchelovodstvo [Feed additives for bees. Apiculture], 7, 14–15 [in Russian].
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28. Kosinov M. V., Kaplunenko V. H. Pat. 38391 UA, MPK C 07 C 51/41 Sposib otrymannia karboksylativ metaliv. Nanotekhnolohiia otrymannia karboksylativ metaliv [Method for metal carboxylates obtaining. Nanotechnology of obtaining metal carboxylates]. Opubl. 12.01.2009, Biul. № 1 [in Ukrainian].
29. Solayman, M., Islam, M. A, Paul, S., Ali Y., Khalil, M. I., Alam, N. & Gan, S. H. (2016). Physicochemical Properties, Minerals, Trace Elements, and Heavy Metals in Honey of Different Origins, 15, 119–233. doi.org/10.1111/1541-4337.12182
30. Stoika, R. S. (2017). Bahatofunktsionalni nanomaterialy dlia biolohii i medytsyny: molekuliarnyi dyzain, syntez i zastosuvannia [Multifunctional nanomaterias for biology and medicine: molecular design, synthesis and application]. «Naukova dumka»: Kyiv, 363 [in Ukrainian].
31. Turko, Ya. I. (2015). Vplyv nanokobaltu na antyokysliuvalnoi systemy orhanizmu shchuriv za hostroho toksykolohichnoho eksperymentu [Influence of nanocobalt on antioxidant system of rats by acute incisive experiment]. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni Z. S, Hzhytskoho, 17, 1(61), 194–200 [in Ukrainian].
32. Ushkalov, V. O., & Turko, Ya. I. (2016). Stan antyokysliuvalnoi systemy orhanizmu shchuriv za dii nanokobaltu v khronichnomu toksykolohichnomu eksperymenti [Antioxidant system state of the rats organism at action of nanocobalt in chronic toxicological experiment]. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S. Z. Gzhytskoho, 18 (1, 65), 238–243 [in Ukrainian].
33. Vlizlo, V. V., Fedoruk R. S., & Iskra R.Ya. (2018). Biolohichna diia funktsionalnykh nanomaterialiv u riznykh vydiv tvaryn [Biological effect of functional nano-materials in various species of animals]. Visnyk Ahrarnoi Nauky, 96(11), 80–86 [in Ukrainian].
34. Yakubchak, O. M. & Yermak A. V. (2019). Vplyv hermaniiu tsytratu na pokaznyky yakosti ta bezpechnosti medu naturalnoho [The influence of hermanium citrate on the indicators of quality and safety of natural honey] Naukovi dopovidi NUBIP Ukrainy, 2(78), 1–9 [in Ukrainian].
35. Yang, W., Tian, Y., Han, M. & Miao, X. (2017). Longevity extension of worker honey bees (Apis mellifera) by royal jelly optimaldose and active ingredient, 5, 3118; DOI 10.7717/peerj.3118.
36. Zhumadina, Sh. M., Kalashnikova, M. V., Sidorova, K. A., & Pashajan, S. A. (2015). Osobennosti morfofunkcional'noj izmenchivosti pchel [Features of morphofunctional variability of bees]. Pavlodar, Kereku, 150 [in Russian].
37. Zoroddu, M. A., Aaseth, J., Crisponi, G., Medici, S., Peana, M. & Nurchi, V. M. (2019). The essential metals for humans: A brief overview. J. Inorg. Biochem., 195, 120–129.
2. Bilalov, F., Skrebneva, L., Nikitin, O., Shuralev, E., & Mukminov M. (2015). Seasonal Variation in Heavy-Metal Accumulation in Honey Bees as an Indicator of Environmental Pollution. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6(4), 215–221.
3. Brych, O. I., Synetar, E. O., & Kaplunenko, V. H. (2015). Perspektyvy zastosuvannia nanoakvakhelativ metaliv [Metals nanoaquahelates prospects usage]. Dosiahnennia biolohii ta medytsyny, 2(26), 64–66 [in Ukrainian].
4. Cebotari, V., Buzu, I., Gliga, O., & Postolachi, O. (2015). New nutritional supplements for bees during deficient harvesting period. Scientific Papers-Animal Science Series: Lucrări Ştiinţifice – Seria Zootehnie, 67, 73–80.
5. Cebotari, V., Buzu, I., & Toderaş, I. (2015a). Influence of some organic coordination compounds containing Co and Bi on development morph-productive characters of the bee families. International Conference of University of Agronomic Sciences and Veterinary Medicine of Bucharest. Faculty of Animal Science. Scientific papers, Series D. Animal Science, Volume LVIII Ed. „Ceres”, România, Bucharest, 181–188.
6. Cho, J. M., Chae, J., Jeong, S. R., Moon, M. J., Shin, D. Y., & Lee, J. H. (2020). Immune activation of Bio-Germanium in a randomized, double-blind, placebocontrolled clinical trial with 130 human subjects: Therapeutic opportunities from new insights. PloS one, 15(10). doi:10.1371/journal.pone.0240358.
7. Chunjiang, T., Lu, X., & Wenlie C. (2015). Germanium in ginseng is low and causes no sodium and water retention or renal toxicity in the diuretic-resistant rats. Experimental Biology and Medicine, 240(11), 1505–1512.
8. DeGrandi-Hoffman, G., Gage, S. L, Corby-Harris, V., Carroll, M., Chambers, M., & Graham, H. (2018). Connecting the nutrient composition of seasonal pollens with changing nutritional needs of honey bee (Apis mellifera L.) colonies. Journal Insect. Physiology, 09, 114–124.
9. Dolaychuk, O. P., Fedoruk, R. S., Kovalchuk, I. I., & Kropyvka, S. I. (2015). Рhysiological and biochemical processes in the organisms of rats when feeding them with different amounts of germanium citrate. Biolohiia tvaryn, 17(2), 50–56 [in Ukrainian].
10. Fedoruk, R. S., Dolaychuk, O. P., Kovalchuk, I. I., & Tsap, M. M. (2015). Reactions of physiological systems rats’ organism by watering them low and high doses Germanium «nanoaquacitrate». Agriculture science and practice, 2(3), 15–21 [in Ukrainian].
11. Fedoruk, R. S., Tesarivska, U. I., Khrabko, M. I., & Tsap, M.M. (2017). Growth and development of the organism and immunophysiological indices of blood of mail F2 rats, affected by different doses of nanogermanium citrate. Agricultural Science and Practice, 4(2), 14–22 [in Ukrainian].
12. Fedoruk, R. S., Tesarivska, U. I., Khrabko, M. I., Tsap, M. M., & Denys, G. G. (2018). Influence of dispensing different doses of germanium citrate on the content of trace elements in tissues and organs of male rats F2. Agricultural Science and Practice, 5(3), 40–46 [in Ukrainian].
13. Fedoruk, R. S., Pashhenko, A. G., Koval'chuk, I. I., & Romaniv, L. I. (2016). Intensivnost' otkladyvanija jaic pchelinymi matkami v vesennij period pri skarmlivanii ih sem'jam citratov Co i Ni s saharnym siropom [Intensity of laying eggs by queen bees in spring when feeding Co and Ni citrates with sugar syrup to their families]. Sollection of works of scientific symposium with international participation ,,Zootechnycal science – an important factor for the european type of the agriculture”. Maximovca, 774–779 [in Russian].
14. Fu, Z., & Xi, S. (2020). The effects of heavy metals on human metabolism. Toxicol. Mech. Methods, 30, 167–176.
15. Kaplunenko, V. H., Fedoruk, R. S., Kovalchuk, I. I., Pashchenko, A. H., Romaniv, L. I., Dvyliuk, I. I., & Kykish, I. B. (2017). Biologic action of citrates of the microelements in melliferous bees in different periods of their lives. Journal of Trace Elements in Medicine and Biology, 41(S1), 64.
16. Kovalchuk, I. I., Kykish, I. B., & Kaplunenko, V. H. (2020). Vplyv tsytrativ mikroelementiv na reproduktyvnu zdatnist bdzholynykh matok [Actual problems of natural sciences: modern scientific discussions] : Collective monograph. Riga, Latvia : “Baltija Publishing”, 87–110.
17. Kovalchuk, I. I., Kaplunenko, V. G., Pashchenko, A. G., Dvylyuk, I. I., & Kykish, I. B. (2017). Trace elements of bees tissues after feeding by citrate-based mineral and hydrocarbon complexes. 33 Joint Annual Meeting of the German Society for Minerals and Trace Elements (GMS) with Zinc-UK «Zinc and other Transition Metals in Health and Disease», 35.
18. Kykish, I. B., Koval'chuk, I. I. & Romaniv L. I. (2017). Vlijanie raznyh doz mikrojelementnogo preparata na vyzhivaemost' medonosnyh pchel [Influence of different doses of a microelement preparation on the survival of honey bees]. Sovremennye problemy veterinarnoj patologii i biotehnologii v agropromyshlennom komplekse: materialy Mezhdunarodnoj nauchno-prakticheskoj konferencii, posvjashhennoj 95–letiju RUP «Institut jeksperemental'noj veterinarii imeni S. N. Vyshelesskogo», Minsk, 16-17 November, 321–324 [in Russian]
19. Li, L., Ruan, T., Lyu, Y. & Wu, B. (2017). Advances in Effect of Germanium or Germanium Compounds on Animals. Journal of Biosciences and Medicines, 05, 56–73. doi:10.4236/jbm.2017.57006.
20. Lu, S. L., Tang, J. X. & Deng, L. S. (1997). Effects of Germanium Citrate on the Immunogenic Function of Mice. Guangdong Trace Elements Science, 4, 21–23.
21. Lukevics, E. & Ignatovich, L. (2003). Biological Activity of Organogermanium Compounds. Cheminform, 34, 279–295.
22. Malavolta, M., & Mocchegiani, E. (2018). Trace elements and minerals in Health and Longevity. Part of the Healthy Ageing and Longevity book series (series ed. Suresh I. S. Rattan), 8, 328.
23. Matuszewska, E., Klupczynska, A., Maciotek, K. Kokot, Z. I., & Matysiak J. (2021). Multielemental Analysis of Bee Pollen, Propolis, and Royal Jelly Collected in West-Central Poland. Molecules, 26(9), 2415. doi.org/10.3390/molecules26092415
24. Nefodova, O. O., Halperin, O. I., & Shatorna, V. F. (2019). The influence of cerium and germanium citrates on the process of embryogenesis of rat on the background of cadmium intoxication. Bulletin of problems biology and medicine, 1(1), (148), 273–278. doi:10.29254/2077-4214-2019-1-1-148-273-278.
25. Niu, Z. Y., Liu, F. Z., Wan, Y. S., Liang, X. & Xie, M. (2001). Effects of Germanium on the Growth of Main Immune Organs in Broilers. Journal of Northwest A & F University (Natural Science Edition), 10, 41–43.
26. Pashajan, A. S., Stolbov, N. M. (2008). Kormovye dobavki dlja pchel. Pchelovodstvo [Feed additives for bees. Apiculture], 7, 14–15 [in Russian].
27. Pashajan, S. A., Sidorova, K. A., & Jurina, T. A. (2021). Nekotorye voprosy povyshenija zhiznestojkosti pchel v uslovijah tehnogeneza [Optimization of viability indicators of bees under technogenesis conditions]. Vestnik Kras.GAU, (3), 88–92. DOI: 10.367181819/4036-2021-3-88-92 [in Russian].
28. Kosinov M. V., Kaplunenko V. H. Pat. 38391 UA, MPK C 07 C 51/41 Sposib otrymannia karboksylativ metaliv. Nanotekhnolohiia otrymannia karboksylativ metaliv [Method for metal carboxylates obtaining. Nanotechnology of obtaining metal carboxylates]. Opubl. 12.01.2009, Biul. № 1 [in Ukrainian].
29. Solayman, M., Islam, M. A, Paul, S., Ali Y., Khalil, M. I., Alam, N. & Gan, S. H. (2016). Physicochemical Properties, Minerals, Trace Elements, and Heavy Metals in Honey of Different Origins, 15, 119–233. doi.org/10.1111/1541-4337.12182
30. Stoika, R. S. (2017). Bahatofunktsionalni nanomaterialy dlia biolohii i medytsyny: molekuliarnyi dyzain, syntez i zastosuvannia [Multifunctional nanomaterias for biology and medicine: molecular design, synthesis and application]. «Naukova dumka»: Kyiv, 363 [in Ukrainian].
31. Turko, Ya. I. (2015). Vplyv nanokobaltu na antyokysliuvalnoi systemy orhanizmu shchuriv za hostroho toksykolohichnoho eksperymentu [Influence of nanocobalt on antioxidant system of rats by acute incisive experiment]. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni Z. S, Hzhytskoho, 17, 1(61), 194–200 [in Ukrainian].
32. Ushkalov, V. O., & Turko, Ya. I. (2016). Stan antyokysliuvalnoi systemy orhanizmu shchuriv za dii nanokobaltu v khronichnomu toksykolohichnomu eksperymenti [Antioxidant system state of the rats organism at action of nanocobalt in chronic toxicological experiment]. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S. Z. Gzhytskoho, 18 (1, 65), 238–243 [in Ukrainian].
33. Vlizlo, V. V., Fedoruk R. S., & Iskra R.Ya. (2018). Biolohichna diia funktsionalnykh nanomaterialiv u riznykh vydiv tvaryn [Biological effect of functional nano-materials in various species of animals]. Visnyk Ahrarnoi Nauky, 96(11), 80–86 [in Ukrainian].
34. Yakubchak, O. M. & Yermak A. V. (2019). Vplyv hermaniiu tsytratu na pokaznyky yakosti ta bezpechnosti medu naturalnoho [The influence of hermanium citrate on the indicators of quality and safety of natural honey] Naukovi dopovidi NUBIP Ukrainy, 2(78), 1–9 [in Ukrainian].
35. Yang, W., Tian, Y., Han, M. & Miao, X. (2017). Longevity extension of worker honey bees (Apis mellifera) by royal jelly optimaldose and active ingredient, 5, 3118; DOI 10.7717/peerj.3118.
36. Zhumadina, Sh. M., Kalashnikova, M. V., Sidorova, K. A., & Pashajan, S. A. (2015). Osobennosti morfofunkcional'noj izmenchivosti pchel [Features of morphofunctional variability of bees]. Pavlodar, Kereku, 150 [in Russian].
37. Zoroddu, M. A., Aaseth, J., Crisponi, G., Medici, S., Peana, M. & Nurchi, V. M. (2019). The essential metals for humans: A brief overview. J. Inorg. Biochem., 195, 120–129.
Published
2021-11-29
How to Cite
Kovalchuk, I. I., Kykish, I. B., Fedoruk, R. S., & Tsap, M. M. (2021). MICROELEMENTS OF BEE TISSUES FOR FEEDING WITH NANOTECHNOLOGICAL COBALT AND GERMANIUM CITRATES. Bulletin of Sumy National Agrarian University. The Series: Veterinary Medicine, (3 (54), 31-38. https://doi.org/10.32845/bsnau.vet.2021.3.5
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