SELECTION OF SUBSTRATES AND MICROBIOLOGICAL PREPARATIONS FOR EFFECTIVE CULTIVATION OF LEEK CASSETTE SEEDLINGS FOR THE CONDITIONS OF THE FOREST-STEPPE OF UKRAINE
Abstract
Due to the long growing season, leeks in the forest-steppe of Ukraine are grown mainly by the seedling method. The yield of onion vegetable plants will be higher with the cassette method of growing seedlings. The advantage of cassette seedlings is the possibility of mechanized planting with seedling planting machines. Substrates of different composition, quality and cost are offered for growing vegetable seedlings. A number of physiologically active preparations with different mechanisms of action are popular in vegetable growing, environmentally friendly and affordable. The technology of growing leek cassette seedlings needs to be improved in terms of selecting a substrate for low-capacity cells and optimizing the nutritional conditions of young plants in a limited root volume. The article presents a comparative characterization of the effect of such substrates as universal Generous soil and peat Klasmann TS1 (factor A) on the quality of leek cassette seedlings. The effectiveness of supplementing substrates with microbiological preparations Azotophyte (5 g kg-1) and Mycofriend (5 g kg-1) (factor B) was also evaluated. In the field experiment, the yield of leeks was determined depending on the technology of growing seedlings. Improvement of the substrate for filling the cassettes with microbiological additives Azotophyte and Mycophriend accelerates the formation of leek seedlings by 2–3 days. Based on the studies, it was found that the type of substrate and microbiological preparations significantly influenced the development of the root system of seedlings, the share of which was 29–35 % of the total plant weight. The largest leaf surface (11.32 cm2 plant-1) and total weight (1.73 g plant-1) were obtained when growing cassette seedlings on Klasmann TS1 peat substrate with the introduction of Azotophyte + Mycofriend. However, the type of substrate (factor A) and the interaction of factors A×B did not have a significant effect on such indicators as the raw weight of seedlings on the 10‑th day, the number of leaves, the leaf surface area of seedlings at the time of planting and the marketable weight of leeks. Microbiological additives had a significant and reliable effect on the yield of leeks. The use of Mycofriend was less effective compared to the introduction of Azotophyte into the substrate. On average, over two years, the variation in leek yield was insignificant, with a coefficient of variation – 4 %. As a result, the highest yield was obtained after the combined use of Azotophyte + Mycophriend – 38.3 t ha-1 on the Klasmann TS1 substrate and 37.6 t ha-1 on the Generous Land substrate.
References
2. Bondarenko H.L. & Yakovenko K.I. (2001). Metodyka doslidnoi spravy v ovochivnytstvi i bashtannytstvi. [Research Methodology in case of Vegetables and Melons]. Osnovy, Kharkiv, 234 (in Ukrainian).
3. Ceglie, F. G., Bustamante, M. A., Ben, A. M. & Tittarelli, F. (2015). The challenge of peat substitution in organic seedling production: optimization of growing media formulation through mixture design and response surface analysis. Plos One, 10: e0128600. doi: 10.1371/journal.pone.0128600.
4. Chieb, M. & Gachomo, E. W. (2023). The role of plant growth promoting rhizobacteria in plant drought stress responses. BMC Plant Biol., 23, 407. doi: 10.1186/s12870-023-04403-8.
5. Colo, J., Hajnal-Jafari, T., Durić, S., Stamenov, D., & Hamidović, S. (2014). Plant growth promotion rhizobacteria in onion production. Polish Journal of Microbiology, 63(1), 83–88.
6. Costa, E., Leal, P. A. M., Benett, C. G. S., Benett, K. S. S. & Salamene, L. C. P. (2012). Production of tomato seedlings using different substrates and trays in three protected environments. Eng. Agri., 32, 822–830.
7. Gong, Binbin, Wang, Ning, Zhang, Tiejun, Wu, Xiaolei, Lü, Guiyun, Chu, Xinpei & Gao, Hongbo. (2019). Selection of tomato seedling index based on comprehensive morphology and leaf chlorophyll content. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 35(8): 237–244. doi: 10.11975/j.issn.1002-6819.2019.08.028.
8. Grover, M., Ali, Sk. Z., Sandhya, V., Rasul, A. & Venkateswarlu, B. (2011). Role of microorganisms in adaptation of agriculture crops to abiotic stresses. World J. Microbiol. Biotechnol., 27, 1231–1240. doi: 10.1007/s11274-010-0572-7.
9. Gusatti, M., Zanuzo, M. R., Machado, R. A. F., Vieira, C. V. & Cavalli, E. (2019). Performance of agricultural substrates in the production of lettuce seedlings (Lactuca sativa L.). Scientific Electronic Archives, 12(5), 40–45. doi: 10.36560/1252019807.
10. Hajnal-Jafari, T., Latković, D., Djurić, S., Mrkovački, N. & Najdenovska, O. (2012). The use of Azotobacter in organic maize production. Research. J. Agricultural. Sci., 44(2), 28–32.
11. Han, L. H., Kumi, F., Mao, H. P. & Hu, J. P. (2019). Design and Tests of a Multi-pin Flexible Seedling Pick-up Gripper for Automatic Transplanting. Appl. Eng. Agric., 35(6), 949–957. doi: 10.13031/aea.13426.
12. Han, L., Mo, M., Gao, Y., Ma, H., Xiang, D., Ma, G. & Mao, H. (2022). Effects of New Compounds into Substrates on Seedling Qualities for Efficient Transplanting. Agronomy, 12(5), 983. doi: 10.3390/ agronomy12050983.
13. Karpenko, V., Slobodyanyk, G., Ulianych, O., Schetyna, S., Mostoviak, І. & Voitsekhovskyi, V. (2020). Combined application of microbial preparation, mineral fertilizer and bioadhesive in production of leek. Agronomy Research, 18(1), 148–162. doi: 10.15159/AR.20.014.
14. Kataloh preparativ BTU-tsentr. [Catalog of drugs btu-center]. https://btu-center.com/search/? q=%C0%E7%EE%F2%EE%F4%B3%F2 (in Ukrainian).
15. Kim, Tae-Won, Khakurel, Dhruba, Jeon, Byeong-Gyun & Lee, Sung-Ho. (2021). Effect of environmentally friendly horticultural substrate on onion (Allium cepa L.) seedlings growth. Journal of Agriculture & Life Science, 55, 11–17. doi: 10.14397/jals.2021.55.5.11.
16. Kumar, A. (2001). Influence of nitrogen and potassium application on growth, yield and nutrient uptake by onion (Allium cepa L.). Indian J. Agronomy, 46, 742–746.
17. Kumar, Devendra Kurrey, Kumar, Mahendra, Lahre & Gaurav, S. Pagire. (2018). Effect of Azotobacter on growth and yield of onion (Allium cepa L.) Journal of Pharmacognosy and Phytochemistry, 7(1), 1171–1175.
18. Kumari, M., Swarupa, P., Kesari, K. K. & Kumar A. (2023). Microbial inoculants as plant biostimulants: a review on risk status. Life, 13(1), 12. doi: 10.3390/life13010012.
19. Lim, J. H., Park, S. Y., Chae, W. B., Kim, S. K., Choi, S. K., Yang, E. Y., Lee, M. J., Jang, Y. N., Seo, M. H., & Jang, S. W. (2017). Seedling Conditions for Kimchi Cabbage, Head Lettuce, Cabbage and Broccoli for a Riding-type Transplanter. J. Bios. Eng., 42, 104–111. doi: 10.5307/JBE.2017.42.2.104.
20. Liu, C. J., Duan, Y. L., Jin, R. Z., Han, Y. Y., Hao, J. H. & Fan, S. X. (2018). Spent mushroom substrates as component of growing media for lettuce seedlings. IOP Conf. Series: Earth and Environmental Science, 185, 012016. doi: 10.1088/1755-1315/185/1/012016.
21. Ma, G. X., Mao, H. P., Bu, Q., Han, L. H., Shabbir, A. & Gao, F. (2020). Effect of Compound Biochar Substrate on the Root Growth of Cucumber Plug Seedlings. Agronomy, 10(8), 1080. doi: 10.3390/agronomy10081080.
22. Marcelle, M. Bettoni, Átila, F. Mogor, Volnei, Pauletti, & Nieves, Goicoechea. (2014). Growth and metabolism of onion seedlings as affected by the application of humic substances, mycorrhizal inoculation and elevated CO2. Scientia Horticulturae, 180, 227–235. doi: 10.1016/j.scienta.2014.10.037.
23. Mrkovacki, N., Mezei, S., Kovacev, L., Bjelic, D., Jarak, M., Tyr, S. & Veres, T. (2012). Effect of Azotobacter chroococcum application on production characteristics of sugar beet and microorganisms in sugar beet rhizosphere. Listy cukrovarnicke a reparske, 128, 50–55.
24. Olle, M. (2013). Effect of efficient microorganisms on yield, quality and preservation of vegetables. In: Gardening Forum, 10–13.
25. Pane, C., Spaccini, R., Piccolo, A., Scala, F. & Bonanomi, G. (2011). Compost amendments enhance peat suppressiveness to pythium ultimum, rhizoctonia solani and sclerotinia minor. Biolog. Control, 56, 115–124.
26. Pellejero, G., Miglierina, A., Aschkar, G., Turcato, M. & Jimenez-Ballesta, R. (2016). Use of compost with onion (Allium cepa L.) waste and cattle manure as substrate component for horticultural seedlings. Int. J. Plant Soil Sci., 12(4), 1–10. doi: 10.9734/IJPSS/2016/27347.
27. Prisa, D. (2019b). Improvement quality of aubergine plants with effective microorganisms. Asian Academic Research Journal of Multidisciplinary, 6(3), 1–8.
28. Prisa, D. (2019с). Rhizobacteria and zeolites for overcoming saline stress in the cultivation of succulent plants. The International Journal of Engineering and Science, 8(5), 38–41.
29. Prisa, D. (2019a). Effective microorganisms for the cultivation and qualitative improvement of onion (Allium cepa L.). World Journal of Advanced Research and Reviews, 02(03), 001–007. doi: 10.30574/wjarr.2019.2.3.0038.
30. Rosa, R., Franczuk, J., Zaniewicz-Bajkowska, A., Remiszewski K., Dydiv, O. & Andrejiová, A. (2022). The influence of the biological activator nutrilife on the yield and quality of onions. Bulletin of Lviv National Environmental University. Series "Agronomy", 26, 87–93. doi: 10.31734/agronomy2022.26.087.
31. Sarah, Tietjen, Ines, Graubner & André, Sradnick. (2022). Reducing peat in substrate mixture formulations for press pots using the Taguchi method. Scientia Horticulturae, 295, 110838. doi: 10.1016/j.scienta.2021.110838.
32. Xu, H. L., Wang, R. & Miridha, M. (2001). Effects of Organic Fertilizers and a Microbial Inoculant on Leaf Photosynthesis and fruit Yield and Quality of Tomato plants. Journal of Crop production, 3, 173–182.
33. Yang, S. Y., Moon, Y. H. & Lee, W. H. (2003). The exploitation of new bed soil used by organic inert. Bulletin of the Agricultural College, Chonbuk National University, 34, 29–40.
34. Yasin, M., Jabran, K., Afzal, I., Iqbal, S., Nawaz, M.A., Mahmood, A., Asif, M., Nadeem, M. A., Rahman, Z. U., Adnan, M., Siddiqui, M., Shahid, M. G. & Andreasen, C. (2020). Industrial sawdust waste: An alternative to soilless substrate for garlic (Allium sativum L.). J. Appl. Res. Med. Aromat. Plants, 1–9. doi: 10.1016/j.jarmap.2020.100252.
35. Zamparo, L., Mattiussi, A., Valent, E. & Cattivello, C. (2021). Substrate formulation to improve vegetable seedling quality and environmental sustainability. Acta Hortic., 1305, 63–70. doi: 10.17660/ActaHortic.2021.1305.9.
36. Zhang, X. Y., Zhou, W. K., Chen, Q., Fang, M. M., Zheng, S. S., Ben, S. & Li, C. Y. (2018). Mediator Subunit MED31 is Required for Radial Patterning of Arabidopsis Roots. Proc. Natl Acad. Sci. USA, 115, 5624–5633. doi: 10.1073/pnas.1800592115.