ACCUMULATION OF HEAVY METALS IN SUNFLOWER SEEDLINGS UNDER THE INFLUENCE OF CADMIUM STRESS
Abstract
Steady trend of recent decades is the rapid growth of soil pollution by heavy metals. Metals that do not play a significant role in plant life are particularly dangerous, but they can accumulate in plants and migrate along the food chain. Cadmium is one of such elements, its half-life from the human body is more than 50 years. For breeding programs aimed at creating varieties with low levels of cadmium accumulation, it is important to understand the role of genotype in the processes of metal intake to different parts of plants. An experiment was carried out to study the growth and absorption of trace metallic elements (TMEs) in Sunflower (Helianthus annuus L.) seedlings under Cd stress. The research was done on selection samples of sunflower selected by the method of vegetation evaluation in Sumy NAU in 2018. According to the evaluation results, line 62\3 was characterized by low, and line JB231AC – high level of resistance to cadmium accumulation. In 2020, the seeds of the samples were reseeded at the Henan Institute of Science and Technology. Sunflower seedlings (20-day-old) grown in plastic containers with Hogland’s solution were investigated. From uniformly and well-developed plants were formed 4 samples which were for 7 days in a solution of CdCl2 * 2.5 H20 with a concentration of 0; 25; 50 and 100 μM. Ashing of the samples was performed in nitric acid. Determination of metal content was performed on an atomic adsorption spectrophotometer. In all samples there was a steady trend to decrease plant height and total seedling weight with increasing cadmium concentration. In the case of dried parts of plants, statistically significant, leveling the difference between the samples, the effect of cadmium was detected at a concentration of 50 μm or more for the dry matter of roots and at a concentration of 100 μm for the mass of the aboveground part. It was found that the threshold concentration that provided a significant difference between the cadmium content in the roots and stems was 100 μm. Compared to sample JB231AC, sample 64\2 had a higher cadmium content in the roots by 23 % and 12 % in the aboveground part. With respect to Li, Ni, and Sr, a significant difference between the samples was mostly observed at a concentration of 25 and 50 μM. The accumulation concentration of lithium (Li) and nickel (Ni) in high Cd variety 62\3 was lower than that in low Cd variety JB231AC. The Li and strontium (Sr) concentration in the root were similar to that in the aboveground part under Cd stress, while Ni accumulated in root of JB231AC in slightly higher concentrations than that in aboveground part. The presence of an inverse relationship between the concentration of cadmium and the concentrations of lithium and nickel may indicate both the genotypic features of their transport and the presence of antagonism between the accumulation of these metals. However, this statement requires further research.
References
2. Alaboudi, K. A., Ahmed, B., & Brodie, G. (2018). Phytoremediation of Pb and Cd contaminated soils by using sunflower (Helianthus annuus) plant. Annals of Agricultural Sciences, 63(1),123–127.
3. Aristide, Y. Y. H., Valery, A. D. P., Chantal, K. A. L., Brou, D., & Kouame, D. B. (2021). Assessment of the Health Risks Associated with the Ingestion of Heavy Metals (As, Cd, Cr, Pb, Ni, Zn, Hg) in Vegetable Crops in the City of Daloa (Ivory Coast). American Journal of Applied Chemistry, 9(4).
4. Bán, R., Kovács, A., Nisha, N., Pálinkás, Z., Zalai, M., Yousif, A. I. A., & Körösi, K. (2021). New and High Virulent Pathotypes of Sunflower Downy Mildew (Plasmopara halstedii) in Seven Countries in Europe. J Fungi (Basel), 7(7), 549–549. doi: 10.3390/jof7070549
5. Bashir, S., Qayyum, M. A., Husain, A., Bakhsh, A., Ahmed, N., Hussain, M. B., Elshikh, M. S., Alwahibi, M. S., Almunqedhi, B. M. A., Hussain, R., Wang, Y. F., Zhou, Y., & Diao, Z. H. (2021). Efficiency of different types of biochars to mitigate Cd stress and growth of sunflower (Helianthus L.) in wastewater irrigated agricultural soil. Saudi J Biol Sci, 28, 2453–2459.
6. Belhaj, D., Elloumi, N., Jerbi, B., Zouari, M., Abdallah, F. B., Ayadi, H., & Kallel, M. (2016). Effects of sewage sludge fertilizer on heavy metal accumulation and consequent responses of sunflower (Helianthus annuus). Environ Sci Pollut Res Int, 23, 20168–20177. doi: 10.1007/s11356-016-7193-0
7. Chen, F., Wang, F., Wu, F., Mao, W., Zhang, G., & Zhou, M. (2010). Modulation of exogenous glutathione in antioxidant defense system against Cd stress in the two barley genotypes differing in Cd tolerance. Plant Physiol Biochem, 48, 663–672. doi: 10.1016/j.plaphy.2010.05.001
8. Chen, Y. P., Chen, D., & Liu, Q. (2017). Exposure to a magnetic field or laser radiation ameliorates effects of Pb and Cd on physiology and growth of young wheat seedlings. J Photochem Photobiol B 169, 171-177. https://doi.org/10.1016/j.jphotobiol.2017.03.012.
9. Cornu, J. Y., Bakoto, R., Bonnard, O., Bussière, S., Coriou, C., Sirguey, C., Sterckeman, T., Thunot, S., Visse, M. I., & Nguyen, C. (2016). Cadmium uptake and partitioning during the vegetative growth of sunflower exposed to low Cd concentrations in hydroponics. Plant and Soil, 404(1–2), 263–275. Doi: 10.1007/s11104-016-2839-8
10. Cornu, J. Y., Bussière, S., Coriou, C., Robert, T., Maucourt, M., Deborde, C., Moing, A., & Nguyen, C. (2020). Changes in plant growth, Cd partitioning and xylem sap composition in two sunflower cultivars exposed to low Cd concentrations in hydroponics. Ecotoxicol Environ Saf, 205, 111145. doi: 10.1016/j.ecoenv.2020.111145
11. Dakak, R. A. E., & Hassan, I. A. (2020). The Alleviative Effects of Salicylic Acid on Physiological Indices and Defense Mechanisms of Maize (Zea Mays L. Giza 2) Stressed with Cadmium. Environmental Processes: An International Journal, 7. (3),873–884. doi: 10.1007/s40710-020-00448-1
12. Fan, J. L., Wei, X. Z., Wan, L. C., Zhang, L. Y., Zhao, X. Q., Liu, W. Z., Hao, H. Q., & Zhang, H. Y. (2011). Disarrangement of actin filaments and Ca²⁺ gradient by CdCl₂ alters cell wall construction in Arabidopsis thaliana root hairs by inhibiting vesicular trafficking. J Plant Physiol, 168, 1157–1167. doi: 10.1016/j.jplph.2011.01.031
13. Han, Y., Wu, M., Hao, L., & Yi, H. (2018). Sulfur dioxide derivatives alleviate cadmium toxicity by enhancing antioxidant defence and reducing Cd(2+) uptake and translocation in foxtail millet seedlings. Ecotoxicol Environ Saf, 157, 207–215. doi: 10.1016/j.ecoenv.2018.03.084
14. Hawrylak-Nowak, B., Dresler, S., & Matraszek, R. (2015). Exogenous malic and acetic acids reduce cadmium phytotoxicity and enhance cadmium accumulation in roots of sunflower plants. Plant Physiol Biochem, 94, 225–234. doi: 10.1016/j.plaphy.2015.06.012
15. Issam, S., Wahbi, D., & Yacine, C. (2015). Selenium Treatment Alleviated Oxidative Alteration Generated by Cadmium in Sunflower Roots. British Biotechnology Journal, 5(4). doi: 10.9734/BBJ/2015/14802
16. Jaouani, K., Karmous, I., Ostrowski, M., Ferjani, E. E., Jakubowska, A., & Chaoui, A. (2018). Cadmium effects on embryo growth of pea seeds during germination: Investigation of the mechanisms of interference of the heavy metal with protein mobilization-related factors. J Plant Physiol, 226, 64–76. doi: 10.1016/j.jplph.2018.02.009
17. Jarrah, M., Ghasemi-fasaei, R., Ronaghi, A., Zarei, M., & Mayel, S. (2019). Enhanced Ni phytoextraction by effectiveness of chemical and biological amendments in sunflower plant grown in Ni-polluted soils. Chemistry and Ecology, 35(8),732–745.
18. Khadija, J., Inès, K., Maciej, O., Ezzedine El, F., Anna, J., & Abdelilah, C. (2018). Cadmium effects on embryo growth of pea seeds during germination: Investigation of the mechanisms of interference of the heavy metal with protein mobilization-related factors. Journal of Plant Physiology, 226, 64–76. doi: 10.1080/02757540.2019.1644325
19. Li, Q., Wang, G., Wang, Y., Dan, Y., Guan, C., & Ji, J. (2019). Foliar application of salicylic acid alleviate the cadmium toxicity by modulation the reactive oxygen species in potato. Ecotoxicol Environ Saf, 172, 317–325. doi: 10.1016/j.ecoenv.2019.01.078.
20. Li, X., Zhang, L., Li, Y., Ma, L., Ning, B., & Ma, C. (2012). Changes in photosynthesis, antioxidant enzymes and lipid peroxidation in soybean seedlings exposed to UV-B radiation and/or Cd. Plant and Soil, 352, 377–387. doi: 10.1007/s11104-011-1003-8
21. Mahardika, G., Rinanti, A., & Fachrul, M. F. (2018). Phytoremediation of heavy metal copper (Cu2+) by sunflower (Helianthus annuus L.). IOP Conference Series: Earth and Environmental Science, 106. doi: 10.1088/1755-1315/106/1/012120
22. Maria Celeste, D., Cristina, M., José, M.-P., Carlos, C., Berta, G., & Conceição, S. (2013). Cadmium toxicity affects photosynthesis and plant growth at different levels. Acta Physiologiae Plantarum, 35, 1281–1289. doi: 10.1007/s11738-012-1167-8
23. Prerna, S., Indra, S. & Kavita, S. (2020). Alterations in antioxidative machinery and growth parameters upon application of nitric oxide donor that reduces detrimental effects of cadmium in rice seedlings with increasing days of growth. South African Journal of Botany, 131(C),283–294. doi: 10.1016/j.sajb.2020.02.022
24. Reyes-Hinojosa, D., Lozada-Pérez, C. A., Zamudio Cuevas, Y., López-Reyes, A., Martínez-Nava, G., Fernández-Torres, J., Olivos-Meza, A., L&a-Solis, C., Gutiérrez-Ruiz, M. C., Rojas Del Castillo, E., & Martínez-Flores, K. (2019). Toxicity of cadmium in musculoskeletal diseases. Environ Toxicol Pharmacol, 72, 103219. doi: 10.1016/j.etap.2019.103219
25. Robert, M., & Stengel, P. (1999). Soils and agriculture: soil resource, quality and degradation processes. A world, European and French prospect. Cahiers Detudes Et De Recherches Francophones Agricultures, 8, 301–308.
26. Silveira, R. l. F. Í. H., Jos, L., Arajo, P. F. í., & Ferracci, A. L. S. R. (2021). Photosynthetic Parameters and Growth of Rice, Lettuce, Sunflower and Tomato in an Entisol as Affected by Soil Acidity and Bioaccumulation of Ba, Cd, Cu, Ni, and Zn. Archives of environmental contamination and toxicology. doi: 10.1007/s00244-021-00850-2
27. Singh, A., & Prasad, S. M. (2014). Effect of agro-industrial waste amendment on Cd uptake in Amaranthus caudatus grown under contaminated soil: an oxidative biomarker response. Ecotoxicol Environ Saf, 100, 105–113. doi: 10.1016/j. ecoenv.2013.09.005.
28. Templeton, D. M., & Liu, Y. (2010). Multiple roles of cadmium in cell death and survival. Chem Biol Interact, 188, 267–275. doi: 10.1016/j.cbi.2010.03.040
29. Zaichick, V. & Zaichick, S. (2014). Relations of the Al, B, Ba, Br, Ca, Cl, Cu, Fe, K, Li, Mg, Mn, Na, P, S, Si, Sr, and Zn mass fractions to morphometric parameters in pediatric and nonhyperplastic young adult prostate glands. Biometals: an international journal on the role of metal ions in biology, biochemistry, and medicine, 27(2), 333–348. doi: 10.1007/s10534-014-9716-9
30. Wang, C., Zhang, Y., Liu, Y., Xu, H., Zhang, T., Hu, Z., Lou, L., & Cai, Q. (2020). Ectopic expression of wheat aquaglyceroporin TaNIP2;1 alters arsenic accumulation and tolerance in Arabidopsis thaliana. Ecotoxicology and Environmental Safety, 205, 111131. doi: 10.1016/j.ecoenv.2020.111131
31. Wu, Z., Zhao, X., Sun, X., Tan, Q., Tang, Y., Nie, Z., Qu, C., Chen, Z., & Hu, C. (2015). Antioxidant enzyme systems and the ascorbate-glutathione cycle as contributing factors to cadmium accumulation and tolerance in two oilseed rape cultivars (Brassica napus L.) under moderate cadmium stress. Chemosphere, 138, 526–36. doi: 10.1016/j.chemosphere.2015.06.080
32. Yan, Y., Sun, Q., Yang, J., Zhang, X., & Guo, B. (2021). Source attributions of Cadmium contamination in rice grains by Cadmium isotope composition analysis: A field study. Ecotoxicol Environ Saf, 210, 111865. doi: 10.1016/j.ecoenv.2020.111865.
33. Zaichick, V., Zaichick, S., Karandashev, V., & Nosenko, S. (2009). The Effect of Age and Gender on Al, B, Ba, Ca, Cu, Fe, K, Li, Mg, Mn, Na, P, S, Sr, V, and Zn Contents in Rib Bone of Healthy Humans. Biological Trace Element Research, 129(1–3),107–115. doi: 10.1007/s12011-008-8302-9.
34. Zhou, J., Chen, L. H., Peng, L., Luo, S., & Zeng, Q. R. (2020). Phytoremediation of heavy metals under an oil crop rotation and treatment of biochar from contaminated biomass for safe use. Chemosphere, 247, 125856. doi: 10.1016/j.chemosphere.2020.125856
35. Marguí, E., Queralt, I. & Hidalgo, M (2009). Application of X-ray fluorescence spectrometry to determination and quantitation of metals in vegetal material. TrAC Trends in Analytical Chemistry, 28(3), 362–372. doi: 0.1016/j.trac.2008.11.011
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